EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of Coxsackievirus A10 entry using the two-in-one attachment and uncoating receptor KRM1.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 117, Issue 31, Page 18711-18718, Year 2020
掲載日	2020年8月4日
著者	Yingzi Cui / Ruchao Peng / Hao Song / Zhou Tong / Xiao Qu / Sheng Liu / Xin Zhao / Yan Chai / Peiyi Wang / George F Gao / Jianxun Qi /
PubMed 要旨	KREMEN1 (KRM1) has been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD), which poses a great threat to infants globally. ...KREMEN1 (KRM1) has been identified as a functional receptor for Coxsackievirus A10 (CV-A10), a causative agent of hand-foot-and-mouth disease (HFMD), which poses a great threat to infants globally. However, the underlying mechanisms for the viral entry process are not well understood. Here we determined the atomic structures of different forms of CV-A10 viral particles and its complex with KRM1 in both neutral and acidic conditions. These structures reveal that KRM1 selectively binds to the mature viral particle above the canyon of the viral protein 1 (VP1) subunit and contacts across two adjacent asymmetry units. The key residues for receptor binding are conserved among most KRM1-dependent enteroviruses, suggesting a uniform mechanism for receptor binding. Moreover, the binding of KRM1 induces the release of pocket factor, a process accelerated under acidic conditions. Further biochemical studies confirmed that receptor binding at acidic pH enabled CV-A10 virion uncoating in vitro. Taken together, these findings provide high-resolution snapshots of CV-A10 entry and identify KRM1 as a two-in-one receptor for enterovirus infection.
リンク	Proc Natl Acad Sci U S A / PubMed:32690697 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 3.6 Å
構造データ	30253 7bzn EMDB-30253, PDB-7bzn: Cryo-EM structure of mature Coxsackievirus A10 at pH 7.4 手法: EM (単粒子) / 解像度: 3.1 Å 30254 7bzo EMDB-30254, PDB-7bzo: Cryo-EM structure of mature Coxsackievirus A10 at pH 5.5 手法: EM (単粒子) / 解像度: 3.2 Å 30259 7bzt EMDB-30259, PDB-7bzt: Cryo-EM structure of mature Coxsackievirus A10 in complex with KRM1 at pH 7.4 手法: EM (単粒子) / 解像度: 3.0 Å 30260 7bzu EMDB-30260, PDB-7bzu: Cryo-EM structure of mature Coxsackievirus A10 in complex with KRM1 at pH 5.5 手法: EM (単粒子) / 解像度: 3.0 Å 30287 7c4t EMDB-30287, PDB-7c4t: Cryo-EM structure of A particle Coxsackievirus A10 at pH 7.4 手法: EM (単粒子) / 解像度: 3.6 Å 30290 7c4w EMDB-30290, PDB-7c4w: Cryo-EM structure of A particle Coxsackievirus A10 at pH 5.5 手法: EM (単粒子) / 解像度: 3.4 Å 30291 7c4y EMDB-30291, PDB-7c4y: Cryo-EM structure of empty Coxsackievirus A10 at pH 7.4 手法: EM (単粒子) / 解像度: 3.5 Å 30292 7c4z EMDB-30292, PDB-7c4z: Cryo-EM structure of empty Coxsackievirus A10 at pH 5.5 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-SPH: SPHINGOSINE ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	coxsackievirus a10 (コクサッキーウイルス) homo sapiens (ヒト)
キーワード	VIRUS / Picornavirus / Coxsackievirus A10 / pH 7.4 / mature particle / pH 5.5 / KRM1 / complex / A particle / empty particle