+検索条件
-Structure paper
タイトル | Structure of the yeast Swi/Snf complex in a nucleosome free state. |
---|---|
ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 3398, Year 2020 |
掲載日 | 2020年7月7日 |
![]() | Chengcheng Wang / Zhouyan Guo / Xiechao Zhan / Fenghua Yang / Mingxuan Wu / Xiaofeng Zhang / ![]() |
PubMed 要旨 | SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free ...SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers. |
![]() | ![]() ![]() ![]() |
手法 | EM (単粒子) |
解像度 | 2.89 - 4.1 Å |
構造データ | EMDB-30285, PDB-7c4j: ![]() EMDB-30286: |
由来 |
|
![]() | DNA BINDING PROTEIN / SWI/SNF remodeling / Swi-Snf complex / nucleosome |