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-Structure paper
タイトル | Structural insights into assembly of the ribosomal nascent polypeptide exit tunnel. |
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ジャーナル・号・ページ | Nat Commun, Vol. 11, Issue 1, Page 5111, Year 2020 |
掲載日 | 2020年10月9日 |
著者 | Daniel M Wilson / Yu Li / Amber LaPeruta / Michael Gamalinda / Ning Gao / John L Woolford / |
PubMed 要旨 | The nascent polypeptide exit tunnel (NPET) is a major functional center of 60S ribosomal subunits. However, little is known about how the NPET is constructed during ribosome assembly. We utilized ...The nascent polypeptide exit tunnel (NPET) is a major functional center of 60S ribosomal subunits. However, little is known about how the NPET is constructed during ribosome assembly. We utilized molecular genetics, biochemistry, and cryo-electron microscopy (cryo-EM) to investigate the functions of two NPET-associated proteins, ribosomal protein uL4 and assembly factor Nog1, in NPET assembly. Structures of mutant pre-ribosomes lacking the tunnel domain of uL4 reveal a misassembled NPET, including an aberrantly flexible ribosomal RNA helix 74, resulting in at least three different blocks in 60S assembly. Structures of pre-ribosomes lacking the C-terminal extension of Nog1 demonstrate that this extension scaffolds the tunnel domain of uL4 in the NPET to help maintain stability in the core of pre-60S subunits. Our data reveal that uL4 and Nog1 work together in the maturation of ribosomal RNA helix 74, which is required to ensure proper construction of the NPET and 60S ribosomal subunits. |
リンク | Nat Commun / PubMed:33037216 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.0 - 4.25 Å |
構造データ | EMDB-30170, PDB-7bt6: EMDB-30172: EMDB-30173: EMDB-30174, PDB-7btb: EMDB-30175: EMDB-30176: |
化合物 | ChemComp-GTP: ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | RIBOSOME / Pre-60s / rpl4 |