EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular choreography of primer synthesis by the eukaryotic Pol α-primase.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 3697, Year 2023
掲載日	2023年6月21日
著者	Zuanning Yuan / Roxana Georgescu / Huilin Li / Michael E O'Donnell /
PubMed 要旨	The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20-30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA ...The eukaryotic polymerase α (Pol α) synthesizes an RNA-DNA hybrid primer of 20-30 nucleotides. Pol α is composed of Pol1, Pol12, Primase 1 (Pri1), and Pri2. Pol1 and Pri1 contain the DNA polymerase and RNA primase activities, respectively. It has been unclear how Pol α hands over an RNA primer from Pri1 to Pol1 for DNA primer extension, and how the primer length is defined. Here we report the cryo-EM analysis of yeast Pol α in the apo, primer initiation, primer elongation, RNA primer hand-off from Pri1 to Pol1, and DNA extension states, revealing a series of very large movements. We reveal a critical point at which Pol1-core moves to take over the 3'-end of the RNA from Pri1. DNA extension is limited by a spiral motion of Pol1-core. Since both Pri1 and Pol1-core are flexibly attached to a stable platform, primer growth produces stress that limits the primer length.
リンク	Nat Commun / PubMed:37344454 / PubMed Central
手法	EM (単粒子)
解像度	3.56 - 5.6 Å
構造データ	29345 8foc EMDB-29345, PDB-8foc: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase in Apo state conformation I 手法: EM (単粒子) / 解像度: 3.7 Å 29346 8fod EMDB-29346, PDB-8fod: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in Apo state conformation II 手法: EM (単粒子) / 解像度: 3.8 Å 29347 8foe EMDB-29347, PDB-8foe: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex bound to a template DNA 手法: EM (単粒子) / 解像度: 5.6 Å 29349 8foh EMDB-29349, PDB-8foh: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the RNA synthesis state 手法: EM (単粒子) / 解像度: 4.6 Å 29351 8foj EMDB-29351, PDB-8foj: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the post RNA handoff state 手法: EM (単粒子) / 解像度: 4.8 Å 29352 8fok EMDB-29352, PDB-8fok: Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the DNA elongation state 手法: EM (単粒子) / 解像度: 3.56 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター
由来	saccharomyces cerevisiae (パン酵母) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / DNA polymerase (DNAポリメラーゼ) / primase (DNAプライマーゼ) / DNA replication (DNA複製)