EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Rare ribosomal RNA sequences from archaea stabilize the bacterial ribosome.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 51, Issue 4, Page 1880-1894, Year 2023
掲載日	2023年2月28日
著者	Amos J Nissley / Petar I Penev / Zoe L Watson / Jillian F Banfield / Jamie H D Cate /
PubMed 要旨	The ribosome serves as the universally conserved translator of the genetic code into proteins and supports life across diverse temperatures ranging from below freezing to above 120°C. Ribosomes are ...The ribosome serves as the universally conserved translator of the genetic code into proteins and supports life across diverse temperatures ranging from below freezing to above 120°C. Ribosomes are capable of functioning across this wide range of temperatures even though the catalytic site for peptide bond formation, the peptidyl transferase center, is nearly universally conserved. Here we find that Thermoproteota, a phylum of thermophilic Archaea, substitute cytidine for uridine at large subunit rRNA positions 2554 and 2555 (Escherichia coli numbering) in the A loop, immediately adjacent to the binding site for the 3'-end of A-site tRNA. We show by cryo-EM that E. coli ribosomes with uridine to cytidine mutations at these positions retain the proper fold and post-transcriptional modification of the A loop. Additionally, these mutations do not affect cellular growth, protect the large ribosomal subunit from thermal denaturation, and increase the mutational robustness of nucleotides in the peptidyl transferase center. This work identifies sequence variation across archaeal ribosomes in the peptidyl transferase center that likely confers stabilization of the ribosome at high temperatures and develops a stable mutant bacterial ribosome that can act as a scaffold for future ribosome engineering efforts.
リンク	Nucleic Acids Res / PubMed:36660825 / PubMed Central
手法	EM (単粒子)
解像度	2.21 - 2.39 Å
構造データ	28165 8eiu EMDB-28165: E. coli 70S ribosome with A-loop mutations U2554C and U2555C (Composite Map) PDB-8eiu: E. coli 70S ribosome with A-loop mutations U2554C and U2555C 手法: EM (単粒子) / 解像度: 2.24 Å EMDB-28218: E. coli 70S ribosome with A-loop mutations U2554C and U2555C (50S Focus Refinement) 手法: EM (単粒子) / 解像度: 2.21 Å EMDB-28229: E. coli 70S ribosome with A-loop mutations U2554C and U2555C (30S Focus Refinement) 手法: EM (単粒子) / 解像度: 2.39 Å EMDB-28230: E. coli 70S ribosome with A-loop mutations U2554C and U2555C: A-site tRNA class 2 手法: EM (単粒子) / 解像度: 2.34 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-PAR: PAROMOMYCIN / パロモマイシン / Antimicrobial, 薬剤YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-8AN: 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) / 3′-アミノ-3′-デオキシアデノシン5′-りん酸 ChemComp-SPM: SPERMINE / スペルミン ChemComp-MET: METHIONINE / メチオニン ChemComp-HOH*: WATER
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / RNA / thermophile / A loop