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-Structure paper
タイトル | Bending forces and nucleotide state jointly regulate F-actin structure. |
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ジャーナル・号・ページ | Nature, Vol. 611, Issue 7935, Page 380-386, Year 2022 |
掲載日 | 2022年10月26日 |
著者 | Matthew J Reynolds / Carla Hachicho / Ayala G Carl / Rui Gong / Gregory M Alushin / |
PubMed 要旨 | ATP-hydrolysis-coupled actin polymerization is a fundamental mechanism of cellular force generation. In turn, force and actin filament (F-actin) nucleotide state regulate actin dynamics by tuning F- ...ATP-hydrolysis-coupled actin polymerization is a fundamental mechanism of cellular force generation. In turn, force and actin filament (F-actin) nucleotide state regulate actin dynamics by tuning F-actin's engagement of actin-binding proteins through mechanisms that are unclear. Here we show that the nucleotide state of actin modulates F-actin structural transitions evoked by bending forces. Cryo-electron microscopy structures of ADP-F-actin and ADP-P-F-actin with sufficient resolution to visualize bound solvent reveal intersubunit interfaces bridged by water molecules that could mediate filament lattice flexibility. Despite extensive ordered solvent differences in the nucleotide cleft, these structures feature nearly identical lattices and essentially indistinguishable protein backbone conformations that are unlikely to be discriminable by actin-binding proteins. We next introduce a machine-learning-enabled pipeline for reconstructing bent filaments, enabling us to visualize both continuous structural variability and side-chain-level detail. Bent F-actin structures reveal rearrangements at intersubunit interfaces characterized by substantial alterations of helical twist and deformations in individual protomers, transitions that are distinct in ADP-F-actin and ADP-P-F-actin. This suggests that phosphate rigidifies actin subunits to alter the bending structural landscape of F-actin. As bending forces evoke nucleotide-state dependent conformational transitions of sufficient magnitude to be detected by actin-binding proteins, we propose that actin nucleotide state can serve as a co-regulator of F-actin mechanical regulation. |
リンク | Nature / PubMed:36289330 / PubMed Central |
手法 | EM (らせん対称) / EM (単粒子) |
解像度 | 2.43 - 3.71 Å |
構造データ | EMDB-27114, PDB-8d13: EMDB-27115, PDB-8d14: EMDB-27116, PDB-8d15: EMDB-27117, PDB-8d16: EMDB-27118, PDB-8d17: EMDB-27119, PDB-8d18: |
化合物 | ChemComp-ADP: ChemComp-MG: ChemComp-HOH: ChemComp-PO4: |
由来 |
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キーワード | STRUCTURAL PROTEIN / Cytoskeleton |