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-Structure paper
タイトル | Mapping the deubiquitination module within the SAGA complex. |
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ジャーナル・号・ページ | Structure, Vol. 22, Issue 11, Page 1553-1559, Year 2014 |
掲載日 | 2014年11月4日 |
PubMed 要旨 | The molecular organization of the yeast transcriptional coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA) was analyzed by single-particle electron microscopy. Complete or partial deletion of the ...The molecular organization of the yeast transcriptional coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA) was analyzed by single-particle electron microscopy. Complete or partial deletion of the Sgf73 subunit disconnects the deubiquitination (DUB) module from SAGA and favors in our conditions the cleavage of the C-terminal ends of the Spt7 subunit and the loss of the Spt8 subunit. The structural comparison of the wild-type SAGA with two deletion mutants positioned the DUB module and enabled the fitting of the available atomic models. The localization of the DUB module close to Gcn5 defines a chromatin-binding interface within SAGA, which could be demonstrated by the binding of nucleosome core particles. The TATA-box binding protein (TBP)-interacting subunit Spt8 was found to be located close to the DUB but in a different domain than Spt3, also known to contact TBP. A flexible protein arm brings both subunits close enough to interact simultaneously with TBP. |
リンク | Structure / PubMed:25441028 |
手法 | EM (単粒子) |
解像度 | 25.8 - 30.3 Å |
構造データ | EMDB-2693: EMDB-2694: |
由来 |
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