EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Neutralizing monoclonal antibodies elicited by mosaic RBD nanoparticles bind conserved sarbecovirus epitopes.
ジャーナル・号・ページ	Immunity, Vol. 55, Issue 12, Page 2419-2435.e10, Year 2022
掲載日	2022年12月13日
著者	Chengcheng Fan / Alexander A Cohen / Miso Park / Alfur Fu-Hsin Hung / Jennifer R Keeffe / Priyanthi N P Gnanapragasam / Yu E Lee / Han Gao / Leesa M Kakutani / Ziyan Wu / Harry Kleanthous / Kathryn E Malecek / John C Williams / Pamela J Bjorkman /
PubMed 要旨	Increased immune evasion by SARS-CoV-2 variants of concern highlights the need for new therapeutic neutralizing antibodies. Immunization with nanoparticles co-displaying spike receptor-binding ...Increased immune evasion by SARS-CoV-2 variants of concern highlights the need for new therapeutic neutralizing antibodies. Immunization with nanoparticles co-displaying spike receptor-binding domains (RBDs) from eight sarbecoviruses (mosaic-8 RBD-nanoparticles) efficiently elicits cross-reactive polyclonal antibodies against conserved sarbecovirus RBD epitopes. Here, we identified monoclonal antibodies (mAbs) capable of cross-reactive binding and neutralization of animal sarbecoviruses and SARS-CoV-2 variants by screening single mouse B cells secreting IgGs that bind two or more sarbecovirus RBDs. Single-particle cryo-EM structures of antibody-spike complexes, including a Fab-Omicron complex, mapped neutralizing mAbs to conserved class 1/4 RBD epitopes. Structural analyses revealed neutralization mechanisms, potentials for intra-spike trimer cross-linking by IgGs, and induced changes in trimer upon Fab binding. In addition, we identified a mAb-resembling Bebtelovimab, an EUA-approved human class 3 anti-RBD mAb. These results support using mosaic RBD-nanoparticle vaccination to generate and identify therapeutic pan-sarbecovirus and pan-variant mAbs.
リンク	Immunity / PubMed:36370711 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.2 - 4.1 Å
構造データ	26878 7uz4 EMDB-26878, PDB-7uz4: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-3 手法: EM (単粒子) / 解像度: 3.1 Å 26879 7uz5 EMDB-26879, PDB-7uz5: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-6 手法: EM (単粒子) / 解像度: 3.2 Å 26880 7uz6 EMDB-26880, PDB-7uz6: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-28 手法: EM (単粒子) / 解像度: 2.8 Å 26881 7uz7 EMDB-26881, PDB-7uz7: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-31 手法: EM (単粒子) / 解像度: 2.9 Å 26882 7uz8 EMDB-26882, PDB-7uz8: Structure of the SARS-CoV-2 Omicron BA.1 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-31 手法: EM (単粒子) / 解像度: 3.1 Å 26883 7uz9 EMDB-26883, PDB-7uz9: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, M8a-34 手法: EM (単粒子) / 解像度: 3.5 Å 26884 7uza EMDB-26884, PDB-7uza: Structure of the SARS-CoV-2 S 6P trimer in complex with the mouse antibody Fab fragment, HSW-1 手法: EM (単粒子) / 解像度: 3.1 Å 26885 7uzb EMDB-26885, PDB-7uzb: Structure of the SARS-CoV-2 S S1 doamin in complex with the mouse antibody Fab fragment, HSW-2 手法: EM (単粒子) / 解像度: 4.1 Å PDB-7uzc: Structure of the SARS-CoV-2 RBD in complex with the mouse antibody Fab fragment, M8a-34 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å PDB-7uzd: Structure of the SARS-CoV-2 RBD in complex with the mouse antibody Fab fragment, HSW-2 手法: X-RAY DIFFRACTION / 解像度: 3.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER
由来	severe acute respiratory syndrome coronavirus 2 (ウイルス) mus musculus (ハツカネズミ)
キーワード	IMMUNE SYSTEM/VIRAL PROTEIN / immune system / neutralizing antibody / IMMUNE SYSTEM-VIRAL PROTEIN complex