EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Activation and closed-state inactivation mechanisms of the human voltage-gated K4 channel complexes.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 13, Page 2427-22442.e4, Year 2022
掲載日	2022年7月7日
著者	Wenlei Ye / Hongtu Zhao / Yaxin Dai / Yingdi Wang / Yu-Hua Lo / Lily Yeh Jan / Chia-Hsueh Lee /
PubMed 要旨	The voltage-gated ion channel activity depends on both activation (transition from the resting state to the open state) and inactivation. Inactivation is a self-restraint mechanism to limit ion ...The voltage-gated ion channel activity depends on both activation (transition from the resting state to the open state) and inactivation. Inactivation is a self-restraint mechanism to limit ion conduction and is as crucial to membrane excitability as activation. Inactivation can occur when the channel is open or closed. Although open-state inactivation is well understood, the molecular basis of closed-state inactivation has remained elusive. We report cryo-EM structures of human K4.2 channel complexes in inactivated, open, and closed states. Closed-state inactivation of K4 involves an unprecedented symmetry breakdown for pore closure by only two of the four S4-S5 linkers, distinct from known mechanisms of open-state inactivation. We further capture K4 in a putative resting state, revealing how voltage sensor movements control the pore. Moreover, our structures provide insights regarding channel modulation by KChIP2 and DPP6 auxiliary subunits. Our findings elucidate mechanisms of closed-state inactivation and voltage-dependent activation of the K4 channel.
リンク	Mol Cell / PubMed:35597238 / PubMed Central
手法	EM (単粒子)
解像度	2.24 - 3.02 Å
構造データ	26575 7uk5 EMDB-26575, PDB-7uk5: Human Kv4.2-KChIP2 channel complex in an open state, transmembrane region 手法: EM (単粒子) / 解像度: 2.76 Å 26576 7ukc EMDB-26576, PDB-7ukc: Human Kv4.2-KChIP2 channel complex in an inactivated state, class 1, transmembrane region 手法: EM (単粒子) / 解像度: 3.0 Å 26577 7ukd EMDB-26577, PDB-7ukd: Human Kv4.2-KChIP2 channel complex in an inactivated state, class 2, transmembrane region 手法: EM (単粒子) / 解像度: 2.88 Å 26578 7uke EMDB-26578, PDB-7uke: Human Kv4.2-KChIP2 channel complex in an intermediate state, transmembrane region 手法: EM (単粒子) / 解像度: 3.01 Å 26579 7ukf EMDB-26579, PDB-7ukf: Human Kv4.2-KChIP2 channel complex in a putative resting state, transmembrane region 手法: EM (単粒子) / 解像度: 3.02 Å 26580 7ukg EMDB-26580, PDB-7ukg: Human Kv4.2-KChIP2-DPP6 channel complex in an open state, transmembrane region 手法: EM (単粒子) / 解像度: 2.24 Å 26581 7ukh EMDB-26581, PDB-7ukh: Human Kv4.2-KChIP2-DPP6 channel complex in an open state, intracellular region 手法: EM (単粒子) / 解像度: 2.33 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン ChemComp-HG: Unknown entry / 水銀 ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / potassium channel complex (カリウムチャネル) / MEMBRANE PROTEIN (膜タンパク質)