EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	HIV-1 CD4-binding site germline antibody-Env structures inform vaccine design.
ジャーナル・号・ページ	Nat Commun, Vol. 13, Issue 1, Page 6123, Year 2022
掲載日	2022年10月17日
著者	Kim-Marie A Dam / Christopher O Barnes / Harry B Gristick / Till Schoofs / Priyanthi N P Gnanapragasam / Michel C Nussenzweig / Pamela J Bjorkman /
PubMed 要旨	BG24, a VRC01-class broadly neutralizing antibody (bNAb) against HIV-1 Env with relatively few somatic hypermutations (SHMs), represents a promising target for vaccine strategies to elicit CD4- ...BG24, a VRC01-class broadly neutralizing antibody (bNAb) against HIV-1 Env with relatively few somatic hypermutations (SHMs), represents a promising target for vaccine strategies to elicit CD4-binding site (CD4bs) bNAbs. To understand how SHMs correlate with BG24 neutralization of HIV-1, we report 4.1 Å and 3.4 Å single-particle cryo-EM structures of two inferred germline (iGL) BG24 precursors complexed with engineered Env-based immunogens lacking CD4bs N-glycans. Structures reveal critical Env contacts by BG24 and identify antibody light chain structural features that impede Env recognition. In addition, biochemical data and cryo-EM structures of BG24 variants bound to Envs with CD4bs glycans present provide insights into N-glycan accommodation, including structural modes of light chain adaptations in the presence of the N276 glycan. Together, these findings reveal Env regions critical for germline antibody recognition and potential sites to alter in immunogen design.
リンク	Nat Commun / PubMed:36253376 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.4 - 6.4 Å
構造データ	26490 7ugn EMDB-26490, PDB-7ugn: Cryo-EM structure of BG24 inferred germline Fabs with germline CDR3s and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 - Class 1 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-26491: Cryo-EM structure of BG24 inferred germline Fabs with germline CDR3s and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 - Class 2 手法: EM (単粒子) / 解像度: 3.8 Å 26492 7ugo EMDB-26492, PDB-7ugo: Cryo-EM structure of BG24 inferred germline Fabs with mature CDR3s and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 手法: EM (単粒子) / 解像度: 4.1 Å 26493 7ugp EMDB-26493, PDB-7ugp: Cryo-EM structure of BG24 Fabs with an inferred germline light chain and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 containing the N276 gp120 glycan- Class 1 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-26494: Cryo-EM map of BG24 Fabs with an inferred germline light chain and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 containing the N276 gp120 glycan- Class 2 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-26495: Cryo-EM map of BG24 Fabs with an inferred germline light chain and 10-1074 Fabs in complex with HIV-1 Env immunogen BG505-SOSIPv4.1-GT1 containing the N276 gp120 glycan- Class 3 手法: EM (単粒子) / 解像度: 6.4 Å 26496 7ugq EMDB-26496, PDB-7ugq: Cryo-EM structure of BG24 Fabs with an inferred germline CDRL1 and 10-1074 Fabs in complex with HIV-1 Env 6405-SOSIP.664 手法: EM (単粒子) / 解像度: 3.4 Å PDB-7ugm: Crystal Structure of BG24-iGL CDR3mat Fab 手法: X-RAY DIFFRACTION / 解像度: 1.4 Å
化合物	ChemComp-HOH: WATER / 水 ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス / マンノース
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) homo sapiens (ヒト)
キーワード	IMMUNE SYSTEM (免疫系) / Fab / IgG / BG24 / anti-HIV antibody / bNAb / VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / HIV-1 Env / neutralizing antibody (中和抗体) / CD4 binding site antibody / VIRAL PROTEIN (ウイルスタンパク質) / Germline / precursor / immunogen (免疫原) / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性)