EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structurally derived universal mechanism for the catalytic cycle of the tail-anchored targeting factor Get3.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 29, Issue 8, Page 820-830, Year 2022
掲載日	2022年7月18日
著者	Michelle Y Fry / Vladimíra Najdrová / Ailiena O Maggiolo / Shyam M Saladi / Pavel Doležal / William M Clemons /
PubMed 要旨	Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) ...Tail-anchored (TA) membrane proteins, accounting for roughly 2% of proteomes, are primarily targeted posttranslationally to the endoplasmic reticulum membrane by the guided entry of TA proteins (GET) pathway. For this complicated process, it remains unknown how the central targeting factor Get3 uses nucleotide to facilitate large conformational changes to recognize then bind clients while also preventing exposure of hydrophobic surfaces. Here, we identify the GET pathway in Giardia intestinalis and present the structure of the Get3-client complex in the critical postnucleotide-hydrolysis state, demonstrating that Get3 reorganizes the client-binding domain (CBD) to accommodate and shield the client transmembrane helix. Four additional structures of GiGet3, spanning the nucleotide-free (apo) open to closed transition and the ATP-bound state, reveal the details of nucleotide stabilization and occluded CBD. This work resolves key conundrums and allows for a complete model of the dramatic conformational landscape of Get3.
リンク	Nat Struct Mol Biol / PubMed:35851188
手法	EM (単粒子) / X線回折
解像度	2.23 - 8.46 Å
構造データ	25373 7sq0 EMDB-25373: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 - nucleotide binding domain map PDB-7sq0: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25374: Get3 bound to ADP and the transmembrane domain of the tail-anchored protein Bos1 - overall map 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-25375: Nucleotide-free Get3 in the closed form 手法: EM (単粒子) / 解像度: 8.46 Å PDB-7spy: Get3 bound to ATP from G. intestinalis in the closed form 手法: X-RAY DIFFRACTION / 解像度: 2.23 Å PDB-7spz: Nucleotide-free Get3 in two open forms 手法: X-RAY DIFFRACTION / 解像度: 3 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	saccharomyces cerevisiae (パン酵母) giardia intestinalis (strain atcc 50803 / wb clone c6) (やつひげはらむし) giardia lamblia atcc 50803 (やつひげはらむし)
キーワード	CHAPERONE / Tail-anchored membrane protein targeting Deviant Walker A ATPase targeting factor