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-Structure paper
タイトル | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage. |
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ジャーナル・号・ページ | Sci Adv, Vol. 8, Issue 18, Page eabj9670, Year 2022 |
掲載日 | 2022年5月6日 |
![]() | Vorrapon Chaikeeratisak / Kanika Khanna / Katrina T Nguyen / MacKennon E Egan / Eray Enustun / Emily Armbruster / Jina Lee / Kit Pogliano / Elizabeth Villa / Joe Pogliano / ![]() ![]() |
PubMed 要旨 | Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we ...Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we show that viral particles of nucleus-forming jumbo phage PhiPA3 assemble into a unique structure inside cells we term phage bouquets. We show that after capsids complete DNA packaging at the surface of the phage nucleus, tails assemble and attach to capsids, and these particles accumulate over time in a spherical pattern, with tails oriented inward and the heads outward to form bouquets at specific subcellular locations. Bouquets localize at the same fixed distance from the phage nucleus even when it is mispositioned, suggesting an active mechanism for positioning. These results mark the discovery of a pathway for organizing mature viral particles inside bacteria and demonstrate that nucleus-forming jumbo phages, like most eukaryotic viruses, are highly spatially organized during all stages of their lytic cycle. |
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手法 | EM (トモグラフィー) |
構造データ | ![]() EMDB-25216: Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 4) ![]() EMDB-25217: Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 3) ![]() EMDB-25218: Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 2) ![]() EMDB-25219: Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi showing phage bouquets (same as Fig 4A of the manuscript) |
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