EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Autophosphorylation transforms DNA-PK from protecting to processing DNA ends.
ジャーナル・号・ページ	Mol Cell, Vol. 82, Issue 1, Page 177-189.e4, Year 2022
掲載日	2022年1月6日
著者	Lan Liu / Xuemin Chen / Jun Li / Huaibin Wang / Christopher J Buehl / Noah J Goff / Katheryn Meek / Wei Yang / Martin Gellert /
PubMed 要旨	The DNA-dependent protein kinase (DNA-PK) initially protects broken DNA ends but then promotes their processing during non-homologous end joining (NHEJ). Before ligation by NHEJ, DNA hairpin ends ...The DNA-dependent protein kinase (DNA-PK) initially protects broken DNA ends but then promotes their processing during non-homologous end joining (NHEJ). Before ligation by NHEJ, DNA hairpin ends generated during V(D)J recombination must be opened by the Artemis nuclease, together with autophosphorylated DNA-PK. Structures of DNA-PK bound to DNA before and after phosphorylation, and in complex with Artemis and a DNA hairpin, reveal an essential functional switch. When bound to open DNA ends in its protection mode, DNA-PK is inhibited for cis-autophosphorylation of the so-called ABCDE cluster but activated for phosphorylation of other targets. In contrast, DNA hairpin ends promote cis-autophosphorylation. Phosphorylation of four Thr residues in ABCDE leads to gross structural rearrangement of DNA-PK, widening the DNA binding groove for Artemis recruitment and hairpin cleavage. Meanwhile, Artemis locks DNA-PK into the kinase-inactive state. Kinase activity and autophosphorylation of DNA-PK are regulated by different DNA ends, feeding forward to coordinate NHEJ events.
リンク	Mol Cell / PubMed:34936881 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.4 Å
構造データ	EMDB-25110: CryoEM map of DNA-PK complex VIb with AMPPNP 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-25111: CryoEM map of DNA-PK complex VIIb with AMPPNP 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-25112: CryoEM map of DNA-PK complex VIIa with AMPPNP 手法: EM (単粒子) / 解像度: 4.2 Å 25113 7sgl EMDB-25113, PDB-7sgl: DNA-PK complex of DNA end processing 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-25114: FATKIN domain of the phosphorylated DNA-PKcs 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-25115: KU, Artemis, DNA and DNA-PKcs_Nheat in the NHEJ DNA end processing complex 手法: EM (単粒子) / 解像度: 2.9 Å 25439 7su3 EMDB-25439, PDB-7su3: CryoEM structure of DNA-PK complex VII 手法: EM (単粒子) / 解像度: 3.3 Å 25440 7sud EMDB-25440, PDB-7sud: CryoEM structure of DNA-PK complex VIII 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸 / フィチン酸 ChemComp-ZN*: Unknown entry
由来	homo sapiens (ヒト) Human (ヒト) synthetic construct (人工物) escherichia coli (大腸菌)
キーワード	DNA BINDING PROTEIN/DNA / endonuclease (エンドヌクレアーゼ) / Kinase (キナーゼ) / complex / DNA BINDING PROTEIN (DNA結合タンパク質) / TRANSFERASE (転移酵素) / DNA BINDING PROTEIN-DNA complex / NHEJ (非相同末端結合) / DNA-PK / DNA repair (DNA修復)