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-Structure paper
タイトル | Visualization of distinct substrate-recruitment pathways in the yeast exosome by EM. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 21, Issue 1, Page 95-9102, Year 2014 |
掲載日 | 2013年12月15日 |
著者 | Jun-Jie Liu / Matthew A Bratkowski / Xueqi Liu / Chu-Ya Niu / Ailong Ke / Hong-Wei Wang / |
PubMed 要旨 | The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have ...The eukaryotic exosome is a multisubunit complex typically composed of a catalytically inactive core and the Rrp44 protein, which contains 3'-to-5' exo- and endo-RNase activities. RNA substrates have been shown to be recruited through the core to reach Rrp44's exo-RNase (EXO) site. Using single-particle EM and biochemical analysis, we provide visual evidence that two distinct substrate-recruitment pathways exist. In the through-core route, channeling of the single-stranded substrates from the core to Rrp44 induces a characteristic conformational change in Rrp44. In the alternative direct-access route, this conformational change does not take place, and the RNA substrate is visualized to avoid the core and enter Rrp44's EXO site directly. Our results provide mechanistic explanations for several RNA processing scenarios by the eukaryotic exosome and indicate substrate-specific modes of degradation by this complex. |
リンク | Nat Struct Mol Biol / PubMed:24336220 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 23.0 - 26.0 Å |
構造データ | EMDB-2491: EMDB-2492: EMDB-2493: EMDB-2494: EMDB-2495: EMDB-2496: EMDB-2497: EMDB-2498: EMDB-2499: EMDB-2500: EMDB-2501: EMDB-2502: EMDB-2522: |
由来 |
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