EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 50, Issue 19, Page 10801-10816, Year 2022
掲載日	2022年10月28日
著者	Amal Seffouh / Chirstian Trahan / Tanzila Wasi / Nikhil Jain / Kaustuv Basu / Robert A Britton / Marlene Oeffinger / Joaquin Ortega /
PubMed 要旨	RbgA is an essential protein for the assembly of the 50S subunit in Bacillus subtilis. Depletion of RbgA leads to the accumulation of the 45S intermediate. A strain expressing a RbgA variant with ...RbgA is an essential protein for the assembly of the 50S subunit in Bacillus subtilis. Depletion of RbgA leads to the accumulation of the 45S intermediate. A strain expressing a RbgA variant with reduced GTPase activity generates spontaneous suppressor mutations in uL6. Each suppressor strain accumulates a unique 44S intermediate. We reasoned that characterizing the structure of these mutant 44S intermediates may explain why RbgA is required to catalyze the folding of the 50S functional sites. We found that in the 44S particles, rRNA helices H42 and H97, near the binding site of uL6, adopt a flexible conformation and allow the central protuberance and functional sites in the mutant 44S particles to mature in any order. Instead, the wild-type 45S particles exhibit a stable H42-H97 interaction and their functional sites always mature last. The dependence on RbgA was also less pronounced in the 44S particles. We concluded that the binding of uL6 pauses the maturation of the functional sites, but the central protuberance continues to fold. RbgA exclusively binds intermediates with a formed central protuberance and licenses the folding of the functional sites. Through this mechanism, RbgA ensures that the functional sites of the 50S mature last.
リンク	Nucleic Acids Res / PubMed:35141754 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 4.1 Å
構造データ	24937 7s9u EMDB-24937, PDB-7s9u: 44SR3C ribosomal particle 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-24940: 44SR3C ribosomal particle class 2 手法: EM (単粒子) / 解像度: 3.3 Å 24950 7sae EMDB-24950, PDB-7sae: 44SR70P Class1 ribosomal particle 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-24951: 44SR70P Class2 ribosomal particle 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-24960: The RbgA treated 44SR3C particles_Class1 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-24962: The RbgA treated 44SR3C particles _Class2 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-24965: The 44SR70P particles incubated at 37C for 15 min Class1 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-24966: The 44SR70P particles incubated at 37C for 15 min Class2 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-24967: The 44SR70P particles incubated at 37C for 15 min Class3 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-24971: The RbgA treated 44SR70P particles Class1 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-24972: The RbgA treated 44SR70P particles Class2 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-24973: The RbgA treated 44SR70P particles Class3 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-24975: The RbgA treated 44SR70P particles Class4 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-24977: The 45SRbgA particles incubated at 37C for 15 min Class1 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-24979: The 45SRbgA particles incubated at 37C for 15 min Class2 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-24980: The 70SR70P ribosomes 手法: EM (単粒子) / 解像度: 2.8 Å
由来	bacillus subtilis (枯草菌)
キーワード	RIBOSOME / Ribosome assembly / 50S subunit / RbgA / YlqF / uL6 / YIqF