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-Structure paper
タイトル | Cryo-EM structure of the needle filament tip complex of the type III secretion injectisome. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 118, Issue 44, Year 2021 |
掲載日 | 2021年11月2日 |
著者 | Emily Z Guo / Jorge E Galán / |
PubMed 要旨 | Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md ...Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md multiprotein structure that mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The injectisome is composed of a cytoplasmic sorting platform, and a membrane-embedded needle complex, which is made up of a multiring base and a needle-like filament that extends several nanometers from the bacterial surface. The needle filament is capped at its distal end by another substructure known as the tip complex, which is crucial for the translocation of effector proteins through the eukaryotic cell plasma membrane. Here we report the cryo-EM structure of the Typhimurium needle tip complex docked onto the needle filament tip. Combined with a detailed analysis of structurally guided mutants, this study provides major insight into the assembly and function of this essential component of the type III secretion protein injection machine. |
リンク | Proc Natl Acad Sci U S A / PubMed:34706941 / PubMed Central |
手法 | EM (単粒子) / EM (らせん対称) |
解像度 | 3.7 - 5.0 Å |
構造データ | EMDB-24734: EMDB-24735, PDB-7rye: EMDB-24736: EMDB-24737: |
由来 |
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キーワード | CELL INVASION / protein secretion / bacterial pathogenesis / organelle assembly |