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-Structure paper
タイトル | Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies. |
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ジャーナル・号・ページ | Nature, Vol. 598, Issue 7880, Page 342-347, Year 2021 |
掲載日 | 2021年8月31日 |
![]() | Florian A Lempp / Leah B Soriaga / Martin Montiel-Ruiz / Fabio Benigni / Julia Noack / Young-Jun Park / Siro Bianchi / Alexandra C Walls / John E Bowen / Jiayi Zhou / Hannah Kaiser / Anshu Joshi / Maria Agostini / Marcel Meury / Exequiel Dellota / Stefano Jaconi / Elisabetta Cameroni / Javier Martinez-Picado / Júlia Vergara-Alert / Nuria Izquierdo-Useros / Herbert W Virgin / Antonio Lanzavecchia / David Veesler / Lisa A Purcell / Amalio Telenti / Davide Corti / ![]() ![]() ![]() |
PubMed 要旨 | SARS-CoV-2 infection-which involves both cell attachment and membrane fusion-relies on the angiotensin-converting enzyme 2 (ACE2) receptor, which is paradoxically found at low levels in the ...SARS-CoV-2 infection-which involves both cell attachment and membrane fusion-relies on the angiotensin-converting enzyme 2 (ACE2) receptor, which is paradoxically found at low levels in the respiratory tract, suggesting that there may be additional mechanisms facilitating infection. Here we show that C-type lectin receptors, DC-SIGN, L-SIGN and the sialic acid-binding immunoglobulin-like lectin 1 (SIGLEC1) function as attachment receptors by enhancing ACE2-mediated infection and modulating the neutralizing activity of different classes of spike-specific antibodies. Antibodies to the amino-terminal domain or to the conserved site at the base of the receptor-binding domain, while poorly neutralizing infection of ACE2-overexpressing cells, effectively block lectin-facilitated infection. Conversely, antibodies to the receptor binding motif, while potently neutralizing infection of ACE2-overexpressing cells, poorly neutralize infection of cells expressing DC-SIGN or L-SIGN and trigger fusogenic rearrangement of the spike, promoting cell-to-cell fusion. Collectively, these findings identify a lectin-dependent pathway that enhances ACE2-dependent infection by SARS-CoV-2 and reveal distinct mechanisms of neutralization by different classes of spike-specific antibodies. |
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手法 | EM (単粒子) |
解像度 | 4.6 - 5.3 Å |
構造データ | ![]() EMDB-24607: ![]() EMDB-24608: |
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