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-Structure paper
タイトル | A site of vulnerability at V3 crown defined by HIV-1 bNAb M4008_N1. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 6464, Year 2021 |
掲載日 | 2021年11月9日 |
著者 | Kun-Wei Chan / Christina C Luo / Hong Lu / Xueling Wu / Xiang-Peng Kong / |
PubMed 要旨 | Identification of vulnerable sites defined by broadly neutralizing antibodies (bNAbs) on HIV-1 envelope (Env) is crucial for vaccine design, and we present here a vulnerable site defined by bNAb ...Identification of vulnerable sites defined by broadly neutralizing antibodies (bNAbs) on HIV-1 envelope (Env) is crucial for vaccine design, and we present here a vulnerable site defined by bNAb M4008_N1, which neutralizes about 40% of a tier-2 virus panel. A 3.2 Å resolution cryo-EM structure of M4008_N1 in complex with BG505 DS-SOSIP reveals a large, shallow protein epitope surface centered at the V3 crown of gp120 and surrounded by key glycans. M4008_N1 interacts with gp120 primarily through its hammerhead CDR H3 to form a β-sheet interaction with the V3 crown hairpin. This makes M4008_N1 compatible with the closed conformation of the prefusion Env trimer, and thus distinct from other known V3 crown mAbs. This mode of bNAb approaching the immunogenic V3 crown in the native Env trimer suggests a strategy for immunogen design targeting this site of vulnerability. |
リンク | Nat Commun / PubMed:34753944 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.24 Å |
構造データ | EMDB-24362, PDB-7rai: |
化合物 | ChemComp-NAG: |
由来 |
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キーワード | IMMUNE SYSTEM (免疫系) / HIV (ヒト免疫不全ウイルス) / Envelope (封筒) / bNAb / Complex |