EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state.
ジャーナル・号・ページ	Mol Microbiol, Vol. 89, Issue 5, Page 831-841, Year 2013
掲載日	2013年7月30日
著者	Ariane Briegel / Peter Ames / James C Gumbart / Catherine M Oikonomou / John S Parkinson / Grant J Jensen /
PubMed 要旨	Motile bacteria sense their physical and chemical environment through highly cooperative, ordered arrays of chemoreceptors. These signalling complexes phosphorylate a response regulator which in turn ...Motile bacteria sense their physical and chemical environment through highly cooperative, ordered arrays of chemoreceptors. These signalling complexes phosphorylate a response regulator which in turn governs flagellar motor reversals, driving cells towards favourable environments. The structural changes that translate chemoeffector binding into the appropriate kinase output are not known. Here, we apply high-resolution electron cryotomography to visualize mutant chemoreceptor signalling arrays in well-defined kinase activity states. The arrays were well ordered in all signalling states, with no discernible differences in receptor conformation at 2-3 nm resolution. Differences were observed, however, in a keel-like density that we identify here as CheA kinase domains P1 and P2, the phosphorylation site domain and the binding domain for response regulator target proteins. Mutant receptor arrays with high kinase activities all exhibited small keels and high proteolysis susceptibility, indicative of mobile P1 and P2 domains. In contrast, arrays in kinase-off signalling states exhibited a range of keel sizes. These findings confirm that chemoreceptor arrays do not undergo large structural changes during signalling, and suggest instead that kinase activity is modulated at least in part by changes in the mobility of key domains.
リンク	Mol Microbiol / PubMed:23802570 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	25.0 - 32.0 Å
構造データ	EMDB-2414: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked off - tsr mutant M222R 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-5541: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked off - tsr mutant A413T 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5542: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - low kinase activity state - tsr mutant P221D 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5543: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - low kinase activity state - tsr mutant EEEE 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5545: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - high kinase activity state - tsr mutant QQQQ 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5546: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked on - tsr mutant I241E 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5547: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked on - tsr mutant G235E 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5548: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - high kinase activity state - tsr mutant QEQE 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5549: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked off - tsr mutant A413T, deletion of subdomains P1 and P2 from CheA 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5550: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked off - tsr mutant A413T, subdomain P2 deleted from CheA 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-5716: Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - kinase activity state locked off - tsr variant P221DdeltaP1P2 手法: EM (サブトモグラム平均) / 解像度: 32.0 Å
由来	Escherichia coli (大腸菌)