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-Structure paper
タイトル | Architecture of an RNA polymerase II transcription pre-initiation complex. |
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ジャーナル・号・ページ | Science, Vol. 342, Issue 6159, Page 1238724, Year 2013 |
掲載日 | 2013年11月8日 |
著者 | Kenji Murakami / Hans Elmlund / Nir Kalisman / David A Bushnell / Christopher M Adams / Maia Azubel / Dominika Elmlund / Yael Levi-Kalisman / Xin Liu / Brian J Gibbons / Michael Levitt / Roger D Kornberg / |
PubMed 要旨 | The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron ...The protein density and arrangement of subunits of a complete, 32-protein, RNA polymerase II (pol II) transcription pre-initiation complex (PIC) were determined by means of cryogenic electron microscopy and a combination of chemical cross-linking and mass spectrometry. The PIC showed a marked division in two parts, one containing all the general transcription factors (GTFs) and the other pol II. Promoter DNA was associated only with the GTFs, suspended above the pol II cleft and not in contact with pol II. This structural principle of the PIC underlies its conversion to a transcriptionally active state; the PIC is poised for the formation of a transcription bubble and descent of the DNA into the pol II cleft. |
リンク | Science / PubMed:24072820 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 16.0 Å |
構造データ | EMDB-2394: |
由来 |
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