EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Vascular K channel structural dynamics reveal regulatory mechanism by Mg-nucleotides.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 118, Issue 44, Year 2021
掲載日	2021年11月2日
著者	Min Woo Sung / Zhongying Yang / Camden M Driggers / Bruce L Patton / Barmak Mostofian / John D Russo / Daniel M Zuckerman / Show-Ling Shyng /
PubMed 要旨	Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack ...Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular K channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic K channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational "propeller" and "quatrefoil" geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward K channel activation.
リンク	Proc Natl Acad Sci U S A / PubMed:34711681 / PubMed Central
手法	EM (単粒子)
解像度	3.4 - 4.2 Å
構造データ	23864 7mit EMDB-23864, PDB-7mit: Vascular KATP channel: Kir6.1 SUR2B propeller-like conformation 1 手法: EM (単粒子) / 解像度: 3.4 Å 23880 7mjo EMDB-23880, PDB-7mjo: Vascular KATP channel: Kir6.1 SUR2B quatrefoil-like conformation 1 手法: EM (単粒子) / 解像度: 4.0 Å 23881 7mjp EMDB-23881, PDB-7mjp: Vascular KATP channel: Kir6.1 SUR2B propeller-like conformation 2 手法: EM (単粒子) / 解像度: 4.2 Å 23882 7mjq EMDB-23882, PDB-7mjq: Vascular KATP channel: Kir6.1 SUR2B quatrefoil-like conformation 2 手法: EM (単粒子) / 解像度: 4.2 Å
化合物	ChemComp-K: Unknown entry / カリウムカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-POV: (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate / リン脂質YM / POホスファチジルコリン ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-P5S: O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine / O-(1-O,2-O-ジステアロイル-L-グリセロ-3-ホスホ)セリン / ホスファチジルセリン ChemComp-GBM: 5-chloro-N-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide / グリベンクラミド / 薬剤YM / グリベンクラミド
由来	rattus norvegicus (ドブネズミ)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / KATP (ATP感受性カリウムチャネル) / potassium channel (カリウムチャネル) / vascular (血管)