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-Structure paper
タイトル | Structure of a microtubule-bound axonemal dynein. |
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ジャーナル・号・ページ | Nat Commun, Vol. 12, Issue 1, Page 477, Year 2021 |
掲載日 | 2021年1月20日 |
著者 | Travis Walton / Hao Wu / Alan Brown / |
PubMed 要旨 | Axonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating, a process critical for cellular motility and extracellular fluid flow. Axonemal dyneins are evolutionarily ...Axonemal dyneins are tethered to doublet microtubules inside cilia to drive ciliary beating, a process critical for cellular motility and extracellular fluid flow. Axonemal dyneins are evolutionarily and biochemically distinct from cytoplasmic dyneins that transport cargo, and the mechanisms regulating their localization and function are poorly understood. Here, we report a single-particle cryo-EM reconstruction of a three-headed axonemal dynein natively bound to doublet microtubules isolated from cilia. The slanted conformation of the axonemal dynein causes interaction of its motor domains with the neighboring dynein complex. Our structure shows how a heterotrimeric docking complex specifically localizes the linear array of axonemal dyneins to the doublet microtubule by directly interacting with the heavy chains. Our structural analysis establishes the arrangement of conserved heavy, intermediate and light chain subunits, and provides a framework to understand the roles of individual subunits and the interactions between dyneins during ciliary waveform generation. |
リンク | Nat Commun / PubMed:33473120 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 3.3 - 7.5 Å |
構造データ | EMDB-23082, PDB-7kzm: EMDB-23083, PDB-7kzn: EMDB-23084, PDB-7kzo: |
化合物 | ChemComp-GTP: ChemComp-MG: ChemComp-GDP: |
由来 |
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キーワード | MOTOR PROTEIN / dynein / microtubule / cilia |