EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the bacterial ribosome at 2 Å resolution.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年9月14日
著者	Zoe L Watson / Fred R Ward / Raphaël Méheust / Omer Ad / Alanna Schepartz / Jillian F Banfield / Jamie Hd Cate /
PubMed 要旨	Using cryo-electron microscopy (cryo-EM), we determined the structure of the 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous positioning of protein and RNA residues, ...Using cryo-electron microscopy (cryo-EM), we determined the structure of the 70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous positioning of protein and RNA residues, their detailed chemical interactions, and chemical modifications. Notable features include the first examples of isopeptide and thioamide backbone substitutions in ribosomal proteins, the former likely conserved in all domains of life. The maps also reveal extensive solvation of the small (30S) ribosomal subunit, and interactions with A-site and P-site tRNAs, mRNA, and the antibiotic paromomycin. The maps and models of the bacterial ribosome presented here now allow a deeper phylogenetic analysis of ribosomal components including structural conservation to the level of solvation. The high quality of the maps should enable future structural analyses of the chemical basis for translation and aid the development of robust tools for cryo-EM structure modeling and refinement.
リンク	Elife / PubMed:32924932 / PubMed Central
手法	EM (単粒子)
解像度	1.89 - 2.13 Å
構造データ	22586 7k00 EMDB-22586: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (composite structure) PDB-7k00: Structure of the Bacterial Ribosome at 2 Angstrom Resolution 手法: EM (単粒子) / 解像度: 1.98 Å EMDB-22607: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (70S ribosome) 手法: EM (単粒子) / 解像度: 1.98 Å EMDB-22614: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (50S focused refinement) 手法: EM (単粒子) / 解像度: 1.89 Å EMDB-22632: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (30S focused refinement) 手法: EM (単粒子) / 解像度: 2.04 Å EMDB-22635: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (30S head domain focused refinement) 手法: EM (単粒子) / 解像度: 1.98 Å EMDB-22636: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (30S platform domain focused refinement) 手法: EM (単粒子) / 解像度: 2.01 Å EMDB-22637: Structure of the Bacterial Ribosome at 2 Angstrom Resolution (50S central protuberance focused refinement) 手法: EM (単粒子) / 解像度: 2.13 Å
化合物	ChemComp-PAR: PAROMOMYCIN / パロモマイシン / Antimicrobial, 薬剤YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SPD: SPERMIDINE / スペルミジン ChemComp-SPM: SPERMINE / スペルミン ChemComp-ZN: Unknown entry ChemComp-HOH*: WATER
由来	escherichia coli (大腸菌)
キーワード	RIBOSOME / antibiotics / post-translational modifications / post-transcriptional modifications