EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of radial spokes and associated complexes important for ciliary motility.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 1, Page 29-37, Year 2021
掲載日	2020年12月14日
著者	Miao Gui / Meisheng Ma / Erica Sze-Tu / Xiangli Wang / Fujiet Koh / Ellen D Zhong / Bonnie Berger / Joseph H Davis / Susan K Dutcher / Rui Zhang / Alan Brown /
PubMed 要旨	In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two ...In motile cilia, a mechanoregulatory network is responsible for converting the action of thousands of dynein motors bound to doublet microtubules into a single propulsive waveform. Here, we use two complementary cryo-EM strategies to determine structures of the major mechanoregulators that bind ciliary doublet microtubules in Chlamydomonas reinhardtii. We determine structures of isolated radial spoke RS1 and the microtubule-bound RS1, RS2 and the nexin-dynein regulatory complex (N-DRC). From these structures, we identify and build atomic models for 30 proteins, including 23 radial-spoke subunits. We reveal how mechanoregulatory complexes dock to doublet microtubules with regular 96-nm periodicity and communicate with one another. Additionally, we observe a direct and dynamically coupled association between RS2 and the dynein motor inner dynein arm subform c (IDAc), providing a molecular basis for the control of motor activity by mechanical signals. These structures advance our understanding of the role of mechanoregulation in defining the ciliary waveform.
リンク	Nat Struct Mol Biol / PubMed:33318703 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 6.3 Å
構造データ	22475 7jtk EMDB-22475: Composite cryo-EM density map of the radial spoke 1 isolated from Chlamydomonas reinhardtii PDB-7jtk: Radial spoke 1 isolated from Chlamydomonas reinhardtii 手法: EM (単粒子) / 解像度: 3.2 Å 22480 7jts EMDB-22480: Cryo-EM density map of stalk of radial spoke 1 attached with doublet microtubule from Chlamydomonas reinhardtii PDB-7jts: Stalk of radial spoke 1 attached with doublet microtubule from Chlamydomonas reinhardtii 手法: EM (単粒子) / 解像度: 6.1 Å 22481 7ju4 EMDB-22481: Composite cryo-EM density map of radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule PDB-7ju4: Radial spoke 2 stalk, IDAc, and N-DRC attached with doublet microtubule 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-22482: Cryo-EM density map of on-doublet radial spoke 1 head 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-22483: Cryo-EM density map of on-doublet radial spoke 2 head 手法: EM (単粒子) / 解像度: 4.6 Å EMDB-22486: Cryo-EM density map of RSP1 dimer from two radial spokes 手法: EM (単粒子) / 解像度: 6.3 Å
化合物	ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM
由来	chlamydomonas reinhardtii (クラミドモナス)
キーワード	STRUCTURAL PROTEIN / cilia / native / complex / mechanoregulation / microtubule