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-Structure paper
タイトル | Mesophasic organization of GABA receptors in hippocampal inhibitory synapses. |
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ジャーナル・号・ページ | Nat Neurosci, Vol. 23, Issue 12, Page 1589-1596, Year 2020 |
掲載日 | 2020年11月2日 |
著者 | Yun-Tao Liu / Chang-Lu Tao / Xiaokang Zhang / Wenjun Xia / Dong-Qing Shi / Lei Qi / Cheng Xu / Rong Sun / Xiao-Wei Li / Pak-Ming Lau / Z Hong Zhou / Guo-Qiang Bi / |
PubMed 要旨 | Information processing in the brain depends on specialized organization of neurotransmitter receptors and scaffolding proteins within the postsynaptic density. However, how these molecules are ...Information processing in the brain depends on specialized organization of neurotransmitter receptors and scaffolding proteins within the postsynaptic density. However, how these molecules are organized in situ remains largely unknown. In this study, template-free classification of oversampled sub-tomograms was used to analyze cryo-electron tomograms of hippocampal synapses. We identified type-A GABA receptors (GABARs) in inhibitory synapses and determined their in situ structure at 19-Å resolution. These receptors are organized hierarchically: from GABAR super-complexes with a preferred inter-receptor distance of 11 nm but variable relative angles, through semi-ordered, two-dimensional receptor networks with reduced Voronoi entropy, to mesophasic assembly with a sharp phase boundary. These assemblies likely form via interactions among postsynaptic scaffolding proteins and receptors and align with putative presynaptic vesicle release sites. Such mesophasic self-organization might allow synapses to achieve a 'Goldilocks' state, striking a balance between stability and flexibility and enabling plasticity in information processing. |
リンク | Nat Neurosci / PubMed:33139942 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 19.0 Å |
構造データ | EMDB-22365: EMDB-22366: |
由来 |
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