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-Structure paper
タイトル | Mediator structure and conformation change. |
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ジャーナル・号・ページ | Mol Cell, Vol. 81, Issue 8, Page 1781-11788.e4, Year 2021 |
掲載日 | 2021年4月15日 |
著者 | Heqiao Zhang / Dong-Hua Chen / Rayees U H Mattoo / David A Bushnell / Yannan Wang / Chao Yuan / Lin Wang / Chunnian Wang / Ralph E Davis / Yan Nie / Roger D Kornberg / |
PubMed 要旨 | Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription ...Mediator is a universal adaptor for transcription control. It serves as an interface between gene-specific activator or repressor proteins and the general RNA polymerase II (pol II) transcription machinery. Previous structural studies revealed a relatively small part of Mediator and none of the gene activator-binding regions. We have determined the cryo-EM structure of the Mediator at near-atomic resolution. The structure reveals almost all amino acid residues in ordered regions, including the major targets of activator proteins, the Tail module, and the Med1 subunit of the Middle module. Comparison of Mediator structures with and without pol II reveals conformational changes that propagate across the entire Mediator, from Head to Tail, coupling activator- and pol II-interacting regions. |
リンク | Mol Cell / PubMed:33571424 |
手法 | EM (単粒子) |
解像度 | 3.58 - 4.3 Å |
構造データ | EMDB-22284, PDB-6xp5: EMDB-22397: T module EMDB-22399: |
由来 |
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キーワード | TRANSCRIPTION / Activation / PolII |