EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cross-neutralization of influenza A viruses mediated by a single antibody loop.
ジャーナル・号・ページ	Nature, Vol. 489, Issue 7417, Page 526-532, Year 2012
掲載日	2012年9月27日
著者	Damian C Ekiert / Arun K Kashyap / John Steel / Adam Rubrum / Gira Bhabha / Reza Khayat / Jeong Hyun Lee / Michael A Dillon / Ryann E O'Neil / Aleksandr M Faynboym / Michael Horowitz / Lawrence Horowitz / Andrew B Ward / Peter Palese / Richard Webby / Richard A Lerner / Ramesh R Bhatt / Ian A Wilson /
PubMed 要旨	Immune recognition of protein antigens relies on the combined interaction of multiple antibody loops, which provide a fairly large footprint and constrain the size and shape of protein surfaces that ...Immune recognition of protein antigens relies on the combined interaction of multiple antibody loops, which provide a fairly large footprint and constrain the size and shape of protein surfaces that can be targeted. Single protein loops can mediate extremely high-affinity binding, but it is unclear whether such a mechanism is available to antibodies. Here we report the isolation and characterization of an antibody called C05, which neutralizes strains from multiple subtypes of influenza A virus, including H1, H2 and H3. X-ray and electron microscopy structures show that C05 recognizes conserved elements of the receptor-binding site on the haemagglutinin surface glycoprotein. Recognition of the haemagglutinin receptor-binding site is dominated by a single heavy-chain complementarity-determining region 3 loop, with minor contacts from heavy-chain complementarity-determining region 1, and is sufficient to achieve nanomolar binding with a minimal footprint. Thus, binding predominantly with a single loop can allow antibodies to target small, conserved functional sites on otherwise hypervariable antigens.
リンク	Nature / PubMed:22982990 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.901 - 17.0 Å
構造データ	EMDB-2138: EM map of influenza H1 hemagglutatin in complex with C05 Fab 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-2139: EM map of an influenza H3 hemagglutatin in complex with C05 Fab 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-2140: EM map of influenza H2 hemagglutatin in complex with C05 Fab 手法: EM (単粒子) / 解像度: 17.0 Å PDB-4fnk: Crystal structure of the A/Hong Kong/1/1968 (H3N2) influenza virus hemagglutinin 手法: X-RAY DIFFRACTION / 解像度: 1.901 Å PDB-4fnl: Crystal structure of broadly neutralizing antibody C05 手法: X-RAY DIFFRACTION / 解像度: 2.297 Å PDB-4fp8: Crystal structure of broadly neutralizing antibody C05 bound to H3 influenza hemagglutinin, HA1 subunit 手法: X-RAY DIFFRACTION / 解像度: 2.947 Å PDB-4fqr: Crystal structure of broadly neutralizing antibody C05 bound to H3 influenza hemagglutinin 手法: X-RAY DIFFRACTION / 解像度: 4.1 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-HOH: WATER ChemComp-TRS: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / トリス(ヒドロキシメチル)メチルアンモニウム / pH緩衝剤YM ChemComp-ZN*: Unknown entry
由来	Influenza A virus (A/Solomon Islands/3/2006(H1N1)) (A型インフルエンザウイルス) homo sapiens (ヒト) influenza a virus (A型インフルエンザウイルス) Influenza A virus (A/Japan/305/1957(H2N2)) (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN / viral fusion protein / virus attachment and entry / IMMUNE SYSTEM / Immunoglobulin / immune recognition / VIRAL PROTEIN/IMMUNE SYSTEM / VIRAL PROTEIN-IMMUNE SYSTEM complex