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タイトル | Experimental Phasing of MicroED Data Using Radiation Damage. |
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ジャーナル・号・ページ | Structure, Vol. 28, Issue 4, Page 458-464.e2, Year 2020 |
掲載日 | 2020年4月7日 |
著者 | Michael W Martynowycz / Johan Hattne / Tamir Gonen / |
PubMed 要旨 | We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present ...We previously demonstrated that microcrystal electron diffraction (MicroED) can be used to determine atomic-resolution structures from vanishingly small three-dimensional crystals. Here, we present an example of an experimentally phased structure using only MicroED data. The structure of a seven-residue peptide is solved starting from differences to the diffraction intensities induced by structural changes due to radiation damage. The same wedge of reciprocal space was recorded twice by continuous-rotation MicroED from a set of 11 individual crystals. The data from the first pass were merged to make a "low-dose dataset." The data from the second pass were similarly merged to form a "damaged dataset." Differences between these two datasets were used to identify a single heavy-atom site from a Patterson difference map, and initial phases were generated. Finally, the structure was completed by iterative cycles of modeling and refinement. |
リンク | Structure / PubMed:32023481 / PubMed Central |
手法 | EM (電子線結晶学) |
解像度 | 1.4 Å |
構造データ | EMDB-21202, PDB-6vhb: EMDB-21203, PDB-6vhc: |
化合物 | ChemComp-ZN: ChemComp-ACT: ChemComp-HOH: |
由来 |
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キーワード | PROTEIN FIBRIL / MicroED / damage / phasing / RIP |