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-Structure paper
タイトル | Sub-2 Angstrom resolution structure determination using single-particle cryo-EM at 200 keV. |
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ジャーナル・号・ページ | J Struct Biol X, Vol. 4, Page 100020, Year 2020 |
掲載日 | 2020年2月28日 |
著者 | Mengyu Wu / Gabriel C Lander / Mark A Herzik / |
PubMed 要旨 | Although the advent of direct electron detectors (DEDs) and software developments have enabled the routine use of single-particle cryogenic electron microscopy (cryo-EM) for structure determination ...Although the advent of direct electron detectors (DEDs) and software developments have enabled the routine use of single-particle cryogenic electron microscopy (cryo-EM) for structure determination of well-behaved specimens to high-resolution, there nonetheless remains a discrepancy between the resolutions attained for biological specimens and the information limits of modern transmission electron microscopes (TEMs). Instruments operating at 300 kV equipped with DEDs are the current paradigm for high-resolution single-particle cryo-EM, while 200 kV TEMs remain comparatively underutilized for purposes beyond sample screening. Here, we expand upon our prior work and demonstrate that one such 200 kV microscope, the Talos Arctica, equipped with a K2 DED is capable of determining structures of macromolecules to as high as ∼1.7 Å resolution. At this resolution, ordered water molecules are readily assigned and holes in aromatic residues can be clearly distinguished in the reconstructions. This work emphasizes the utility of 200 kV electrons for high-resolution single-particle cryo-EM and applications such as structure-based drug design. |
リンク | J Struct Biol X / PubMed:32647824 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 1.75 - 2.13 Å |
構造データ | EMDB-21023: Single-particle cryo-EM reconstruction of rabbit muscle aldolase using 200 keV EMDB-21024: Single-particle cryo-EM reconstruction of mouse heavy chain apoferritin at 200 keV |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | LYASE / homo-4-mer / D-fructose / glycolysis / METAL BINDING PROTEIN / OXIDOREDUCTASE / homo-24-mer / storage / globular |