EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.
ジャーナル・号・ページ	Mol Cell, Year 2024
掲載日	2024年5月29日
著者	Francisco Tenjo-Castaño / Nicholas Sofos / Luisa S Stutzke / Piero Temperini / Anders Fuglsang / Tillmann Pape / Pablo Mesa / Guillermo Montoya /
PubMed 要旨	CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opt CRISPR-Cas systems for RNA-guided DNA transposition. CASTs integrate large DNA cargos into the attachment (att) site ...CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opt CRISPR-Cas systems for RNA-guided DNA transposition. CASTs integrate large DNA cargos into the attachment (att) site independently of homology-directed repair and thus hold promise for eukaryotic genome engineering. However, the functional diversity and complexity of CASTs hinder an understanding of their mechanisms. Here, we present the high-resolution cryoelectron microscopy (cryo-EM) structure of the reconstituted ∼1 MDa post-transposition complex of the type V-K CAST, together with different assembly intermediates and diverse TnsC filament lengths, thus enabling the recapitulation of the integration complex formation. The results of mutagenesis experiments probing the roles of specific residues and TnsB-binding sites show that transposition activity can be enhanced and suggest that the distance between the PAM and att sites is determined by the lengths of the TnsB C terminus and the TnsC filament. This singular model of RNA-guided transposition provides a foundation for repurposing the system for genome-editing applications.
リンク	Mol Cell / PubMed:38834066
手法	EM (単粒子)
解像度	2.3 - 3.19 Å
構造データ	19075 8rdu EMDB-19075, PDB-8rdu: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K composite map 手法: EM (単粒子) / 解像度: 2.3 Å 19282 8rkt EMDB-19282, PDB-8rkt: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K Cas12k domain local-refinement map 手法: EM (単粒子) / 解像度: 2.35 Å 19283 8rku EMDB-19283, PDB-8rku: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsC domain local-refinement map 手法: EM (単粒子) / 解像度: 2.3 Å 19284 8rkv EMDB-19284, PDB-8rkv: Conformational Landscape of the Type V-K CRISPR-associated TransposonIntegration Assembly CAST V-K TnsB domain local-refinement map 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-19286: consensus map of the V-K CRISPR-associated Transposon Integration Assembly 手法: EM (単粒子) / 解像度: 3.19 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-HOH*: WATER / 水
由来	scytonema hofmannii (バクテリア)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / CRISPR-associated Transposon genome editing transposition