EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Noncanonical assembly, neddylation and chimeric cullin-RING/RBR ubiquitylation by the 1.8 MDa CUL9 E3 ligase complex.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年4月11日
著者	Daniel Horn-Ghetko / Linus V M Hopf / Ishita Tripathi-Giesgen / Jiale Du / Sebastian Kostrhon / D Tung Vu / Viola Beier / Barbara Steigenberger / J Rajan Prabu / Luca Stier / Elias M Bruss / Matthias Mann / Yue Xiong / Brenda A Schulman /
PubMed 要旨	Ubiquitin ligation is typically executed by hallmark E3 catalytic domains. Two such domains, 'cullin-RING' and 'RBR', are individually found in several hundred human E3 ligases, and collaborate with ...Ubiquitin ligation is typically executed by hallmark E3 catalytic domains. Two such domains, 'cullin-RING' and 'RBR', are individually found in several hundred human E3 ligases, and collaborate with E2 enzymes to catalyze ubiquitylation. However, the vertebrate-specific CUL9 complex with RBX1 (also called ROC1), of interest due to its tumor suppressive interaction with TP53, uniquely encompasses both cullin-RING and RBR domains. Here, cryo-EM, biochemistry and cellular assays elucidate a 1.8-MDa hexameric human CUL9-RBX1 assembly. Within one dimeric subcomplex, an E2-bound RBR domain is activated by neddylation of its own cullin domain and positioning from the adjacent CUL9-RBX1 in trans. Our data show CUL9 as unique among RBX1-bound cullins in dependence on the metazoan-specific UBE2F neddylation enzyme, while the RBR domain protects it from deneddylation. Substrates are recruited to various upstream domains, while ubiquitylation relies on both CUL9's neddylated cullin and RBR domains achieving self-assembled and chimeric cullin-RING/RBR E3 ligase activity.
リンク	Nat Struct Mol Biol / PubMed:38605244
手法	EM (単粒子)
解像度	3.37 - 13.9 Å
構造データ	18214 8q7e EMDB-18214, PDB-8q7e: Structure of CUL9-RBX1 ubiquitin E3 ligase complex - hexameric assembly 手法: EM (単粒子) / 解像度: 4.4 Å 18216 8q7h EMDB-18216, PDB-8q7h: Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused cullin dimer 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-18217: Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused on E2-like density 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-18218: Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated and neddylated conformation - focused dimeric core 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-18220: Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 CPH domain 手法: EM (単粒子) / 解像度: 9.5 Å EMDB-18221: Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 DOC domain 手法: EM (単粒子) / 解像度: 13.7 Å EMDB-18222: Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 ARM9 domain 手法: EM (単粒子) / 解像度: 12.5 Å EMDB-18223: Structure of the hexameric CUL9-RBX1 complex with deletion of CUL9 ARIH-RBR element 手法: EM (単粒子) / 解像度: 13.9 Å 19179 8rhz EMDB-19179, PDB-8rhz: Structure of CUL9-RBX1 ubiquitin E3 ligase complex in unneddylated conformation - symmetry expanded unneddylated dimer 手法: EM (単粒子) / 解像度: 3.37 Å
化合物	ChemComp-ZN: Unknown entry
由来	homo sapiens (ヒト)
キーワード	LIGASE (リガーゼ) / Cullin-RING RBR E3 Ligase