EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Outer membrane protein assembly mediated by BAM-SurA complexes.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 7612, Year 2024
掲載日	2024年9月1日
著者	Katherine L Fenn / Jim E Horne / Joel A Crossley / Nils Böhringer / Romany J Horne / Till F Schäberle / Antonio N Calabrese / Sheena E Radford / Neil A Ranson /
PubMed 要旨	The outer membrane is a formidable barrier that protects Gram-negative bacteria against environmental threats. Its integrity requires the correct folding and insertion of outer membrane proteins ...The outer membrane is a formidable barrier that protects Gram-negative bacteria against environmental threats. Its integrity requires the correct folding and insertion of outer membrane proteins (OMPs) by the membrane-embedded β-barrel assembly machinery (BAM). Unfolded OMPs are delivered to BAM by the periplasmic chaperone SurA, but how SurA and BAM work together to ensure successful OMP delivery and folding remains unclear. Here, guided by AlphaFold2 models, we use disulphide bond engineering in an attempt to trap SurA in the act of OMP delivery to BAM, and solve cryoEM structures of a series of complexes. The results suggest that SurA binds BAM at its soluble POTRA-1 domain, which may trigger conformational changes in both BAM and SurA that enable transfer of the unfolded OMP to the BAM lateral gate for insertion into the outer membrane. Mutations that disrupt the interaction between BAM and SurA result in outer membrane assembly defects, supporting the key role of SurA in outer membrane biogenesis.
リンク	Nat Commun / PubMed:39218969 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 5.3 Å
構造データ	18034 8pz1 EMDB-18034, PDB-8pz1: Wait Complex: Lateral open BAM bound Compact SurA 手法: EM (単粒子) / 解像度: 4.1 Å 18035 8pz2 EMDB-18035, PDB-8pz2: Wait Complex: Lateral open BAM bound Extended SurA 手法: EM (単粒子) / 解像度: 4.2 Å 18045 8pzu EMDB-18045, PDB-8pzu: Wait Complex: BAM bound Darobactin-B and Compact SurA 手法: EM (単粒子) / 解像度: 3.5 Å 18046 8pzv EMDB-18046, PDB-8pzv: Wait Complex: BAM bound Darobactin-B and Extended SurA 手法: EM (単粒子) / 解像度: 2.9 Å 18053 8q0g EMDB-18053, PDB-8q0g: Release Complex: BAM bound EspP and Compact SurA 手法: EM (単粒子) / 解像度: 4.3 Å 18543 8qp5 EMDB-18543, PDB-8qp5: Release Complex: BAM bound EspP (SurA released) 手法: EM (単粒子) / 解像度: 4.4 Å 18562 8qpu EMDB-18562, PDB-8qpu: Release Complex: BAM bound EspP and Extended SurA 手法: EM (単粒子) / 解像度: 5.2 Å 18563 8qpv EMDB-18563, PDB-8qpv: Handover Complex: BAM bound OmpX and extended SurA 手法: EM (単粒子) / 解像度: 4.0 Å 18564 8qpw EMDB-18564, PDB-8qpw: Arrival Complex: Lateral open BAM bound extended SurA plus OmpX 手法: EM (単粒子) / 解像度: 5.3 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER
由来	escherichia coli (大腸菌) photorhabdus heterorhabditis (バクテリア)
キーワード	MEMBRANE PROTEIN / Outer Membrane / Complex / Chaperone / Protein Folding / Inhibitor