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-Structure paper
タイトル | Structural insights into cognate versus near-cognate discrimination during decoding. |
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ジャーナル・号・ページ | EMBO J, Vol. 30, Issue 8, Page 1497-1507, Year 2011 |
掲載日 | 2011年4月20日 |
著者 | Xabier Agirrezabala / Eduard Schreiner / Leonardo G Trabuco / Jianlin Lei / Rodrigo F Ortiz-Meoz / Klaus Schulten / Rachel Green / Joachim Frank / |
PubMed 要旨 | The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near- ...The structural basis of the tRNA selection process is investigated by cryo-electron microscopy of ribosomes programmed with UGA codons and incubated with ternary complex (TC) containing the near-cognate Trp-tRNA(Trp) in the presence of kirromycin. Going through more than 350 000 images and employing image classification procedures, we find ∼8% in which the TC is bound to the ribosome. The reconstructed 3D map provides a means to characterize the arrangement of the near-cognate aa-tRNA with respect to elongation factor Tu (EF-Tu) and the ribosome, as well as the domain movements of the ribosome. One of the interesting findings is that near-cognate tRNA's acceptor stem region is flexible and CCA end becomes disordered. The data bring direct structural insights into the induced-fit mechanism of decoding by the ribosome, as the analysis of the interactions between small and large ribosomal subunit, aa-tRNA and EF-Tu and comparison with the cognate case (UGG codon) offers clues on how the conformational signals conveyed to the GTPase differ in the two cases. |
リンク | EMBO J / PubMed:21378755 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 8.25 - 13.2 Å |
構造データ | |
由来 |
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キーワード | RIBOSOME / translation / ternary complex / tRNA incorporation / cryoEM / near-cognate |