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-Structure paper
| タイトル | Mechanism of mammalian transcriptional repression by noncoding RNA. |
|---|---|
| ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 32, Issue 4, Page 607-612, Year 2025 |
| 掲載日 | 2025年1月6日 |
著者 | Katarína Tlučková / Beata Kaczmarek / Anita Salmazo / Carrie Bernecky / ![]() |
| PubMed 要旨 | Transcription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here ...Transcription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here we present cryogenic-electron microscopy structures of Pol II bound to Alu RNA, which reveal that Alu RNA mimics how DNA and RNA bind to Pol II during transcription elongation. Further, we show how distinct domains of the general transcription factor TFIIF control repressive activity. Together, we reveal how a noncoding RNA can regulate mammalian gene expression. |
リンク | Nat Struct Mol Biol / PubMed:39762629 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.4 - 3.2 Å |
| 構造データ | ![]() EMDB-18367: RNA polymerase II bound to Alu RNA left arm ![]() EMDB-18371: RNA polymerase II bound to minimal Alu RNA right arm in the canonical conformation EMDB-18375, PDB-8qep: EMDB-18376, PDB-8qeq: |
| 化合物 | ![]() ChemComp-ZN: ![]() ChemComp-MG: |
| 由来 |
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キーワード | TRANSCRIPTION / transcription inhibition / noncoding RNA / RNA polymerase II / Alu RNA |
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