EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Genome anchoring, retention, and release by neck proteins of Staphylococcus phage 812.
ジャーナル・号・ページ	Commun Biol, Vol. 9, Issue 1, Page 199, Year 2026
掲載日	2026年1月8日
著者	Zuzana Cieniková / Jiří Nováček / Marta Šiborová / Barbora Popelářová / Tibor Füzik / Tibor Botka / Martin Benešík / Pavol Bárdy / Roman Pantůček / Pavel Plevka /
PubMed 要旨	The virion of Staphylococcus phage 812 is formed by a capsid and a contractile tail joined together by neck proteins. The neck proteins are crucial for virion assembly, DNA packaging, and the ...The virion of Staphylococcus phage 812 is formed by a capsid and a contractile tail joined together by neck proteins. The neck proteins are crucial for virion assembly, DNA packaging, and the regulation of genome release, but their functions are not completely understood. Here, we show that the neck of phage 812 consists of portal, adaptor, stopper, tail terminator, and two types of decoration proteins. A dodecameric DNA-binding site at the surface of the portal complex anchors the phage genome inside the capsid. The adaptor complex induces a local B-to-A form transition of the DNA in the neck channel that could slow or pause genome translocation during ejection. The central channel of a stopper complex that is not attached to the tail terminator complex is closed by gating loops. In contrast, in the phage 812 virion, the gating loops are in an open conformation, and the DNA extends into the tail. The structure of neck proteins is not affected by tail sheath contraction. Therefore, the expulsion of tail tape measure proteins triggers the genome release.
リンク	Commun Biol / PubMed:41507424 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.2 - 8.64 Å
構造データ	18048 8q01 EMDB-18048, PDB-8q01: Neck of phage 812 after tail contraction (C6) 手法: EM (単粒子) / 解像度: 3.58 Å 18065 8q1i EMDB-18065, PDB-8q1i: Neck-tail junction of phage 812 after tail contraction (C6) 手法: EM (単粒子) / 解像度: 3.53 Å 18213 8q7d EMDB-18213, PDB-8q7d: Neck of phage 812 after tail contraction (C12) 手法: EM (単粒子) / 解像度: 3.07 Å 18369 8qek EMDB-18369, PDB-8qek: Neck and tail of phage 812 after tail contraction (composite) 手法: EM (単粒子) / 解像度: 3.6 Å 18372 8qem EMDB-18372, PDB-8qem: Neck channel of phage 812 after tail contraction (C1) 手法: EM (単粒子) / 解像度: 3.96 Å EMDB-18385: Consensus density for neck and tail of phage 812 after tail contraction (C6) 手法: EM (単粒子) / 解像度: 4.23 Å EMDB-18395: Portal and anchor DNA of phage 812 after tail contraction (C1) 手法: EM (単粒子) / 解像度: 3.94 Å 18445 8qje EMDB-18445, PDB-8qje: Neck of phage 812 virion (C12) 手法: EM (単粒子) / 解像度: 4.16 Å 18462 8qkh EMDB-18462, PDB-8qkh: Neck of phage 812 virion (C6) 手法: EM (単粒子) / 解像度: 4.15 Å EMDB-18489: Tail channel of phage 812 virion (C3) 手法: EM (単粒子) / 解像度: 6.56 Å EMDB-18516: Tail channel of phage 812 virion (C1) 手法: EM (単粒子) / 解像度: 8.64 Å 18912 8r5g EMDB-18912, PDB-8r5g: Neck-tail junction of phage 812 virion (C6) 手法: EM (単粒子) / 解像度: 4.28 Å EMDB-18919: Consensus density for neck and tail of phage 812 virion (C6) 手法: EM (単粒子) / 解像度: 4.64 Å 18949 8r69 EMDB-18949, PDB-8r69: Neck and tail of phage 812 virion (composite) 手法: EM (単粒子) / 解像度: 4.3 Å PDB-8qgr: Stopper protein of phage 812 in closed conformation 手法: X-RAY DIFFRACTION / 解像度: 2.2 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-HOH: WATER
由来	staphylococcus phage 812 (ファージ)
キーワード	VIRUS / phage / neck / connector / tail sheath / portal / tail / A-form DNA