EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Concerted transformation of a hyper-paused transcription complex and its reinforcing protein.
ジャーナル・号・ページ	Nat Commun, Vol. 15, Issue 1, Page 3040, Year 2024
掲載日	2024年4月8日
著者	Philipp K Zuber / Nelly Said / Tarek Hilal / Bing Wang / Bernhard Loll / Jorge González-Higueras / César A Ramírez-Sarmiento / Georgiy A Belogurov / Irina Artsimovitch / Markus C Wahl / Stefan H Knauer /
PubMed 要旨	RfaH, a paralog of the universally conserved NusG, binds to RNA polymerases (RNAP) and ribosomes to activate expression of virulence genes. In free, autoinhibited RfaH, an α-helical KOW domain ...RfaH, a paralog of the universally conserved NusG, binds to RNA polymerases (RNAP) and ribosomes to activate expression of virulence genes. In free, autoinhibited RfaH, an α-helical KOW domain sequesters the RNAP-binding site. Upon recruitment to RNAP paused at an ops site, KOW is released and refolds into a β-barrel, which binds the ribosome. Here, we report structures of ops-paused transcription elongation complexes alone and bound to the autoinhibited and activated RfaH, which reveal swiveled, pre-translocated pause states stabilized by an ops hairpin in the non-template DNA. Autoinhibited RfaH binds and twists the ops hairpin, expanding the RNA:DNA hybrid to 11 base pairs and triggering the KOW release. Once activated, RfaH hyper-stabilizes the pause, which thus requires anti-backtracking factors for escape. Our results suggest that the entire RfaH cycle is solely determined by the ops and RfaH sequences and provide insights into mechanisms of recruitment and metamorphosis of NusG homologs across all life.
リンク	Nat Commun / PubMed:38589445 / PubMed Central
手法	EM (単粒子)
解像度	2.6 - 3.4 Å
構造データ	17626 8pdy EMDB-17626, PDB-8pdy: E. coli RNA polymerase paused at ops site 手法: EM (単粒子) / 解像度: 2.8 Å 17632 8pen EMDB-17632, PDB-8pen: fully recruited RfaH bound to E. coli transcription complex paused at ops site (alternative state of RfaH) 手法: EM (単粒子) / 解像度: 3.1 Å 17646 8pfg EMDB-17646: transcription complex paused at ops site and bound to autoinhibited RfaH, not fully complementary scaffold PDB-8pfg: autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter complex), not fully complementary scaffold 手法: EM (単粒子) / 解像度: 3.1 Å 17647 8pfj EMDB-17647, PDB-8pfj: fully recruited RfaH bound to E. coli transcription complex paused at ops site (not fully complementary scaffold; alternative state of RfaH) 手法: EM (単粒子) / 解像度: 3.4 Å 17657 8ph9 EMDB-17657, PDB-8ph9: E. coli RNA polymerase paused at ops site (non-complementary scaffold) 手法: EM (単粒子) / 解像度: 3.0 Å 17668 8phk EMDB-17668: E. coli transcription complex paused at ops site with fully recruited RfaH PDB-8phk: fully recruited RfaH bound to E. coli transcription complex paused at ops site 手法: EM (単粒子) / 解像度: 3.1 Å 17679 8pib EMDB-17679, PDB-8pib: autoinhibited RfaH bound to E. coli transcription complex paused at ops site (encounter complex) 手法: EM (単粒子) / 解像度: 2.6 Å 17681 8pid EMDB-17681, PDB-8pid: backtracked E. coli transcription complex paused at ops site and bound to RfaH 手法: EM (単粒子) / 解像度: 3.0 Å 17685 8pil EMDB-17685, PDB-8pil: E. coli transcription complex paused at ops site and bound to RfaH and NusA 手法: EM (単粒子) / 解像度: 3.2 Å 17686 8pim EMDB-17686, PDB-8pim: fully recruited RfaH bound to E. coli transcription complex paused at ops site (not complementary scaffold) 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	escherichia coli (大腸菌)
キーワード	TRANSCRIPTION / transcription complex / pause at ops site / DNA hairpin / pausing / recruited RfaH / ops hairpin / RfaH / ops / ops site / encounter complex / backtracking / NusA