EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of a sperm-specific solute carrier gated by voltage and cAMP.
ジャーナル・号・ページ	Nature, Vol. 623, Issue 7985, Page 202-209, Year 2023
掲載日	2023年10月25日
著者	Valeria Kalienkova / Martin F Peter / Jan Rheinberger / Cristina Paulino /
PubMed 要旨	The newly characterized sperm-specific Na/H exchanger stands out by its unique tripartite domain composition. It unites a classical solute carrier unit with regulatory domains usually found in ion ...The newly characterized sperm-specific Na/H exchanger stands out by its unique tripartite domain composition. It unites a classical solute carrier unit with regulatory domains usually found in ion channels, namely, a voltage-sensing domain and a cyclic-nucleotide binding domain, which makes it a mechanistic chimera and a secondary-active transporter activated strictly by membrane voltage. Our structures of the sea urchin SpSLC9C1 in the absence and presence of ligands reveal the overall domain arrangement and new structural coupling elements. They allow us to propose a gating model, where movements in the voltage sensor indirectly cause the release of the exchanging unit from a locked state through long-distance allosteric effects transmitted by the newly characterized coupling helices. We further propose that modulation by its ligand cyclic AMP occurs by means of disruption of the cytosolic dimer interface, which lowers the energy barrier for S4 movements in the voltage-sensing domain. As SLC9C1 members have been shown to be essential for male fertility, including in mammals, our structure represents a potential new platform for the development of new on-demand contraceptives.
リンク	Nature / PubMed:37880361 / PubMed Central
手法	EM (単粒子)
解像度	3.05 - 3.74 Å
構造データ	17596 8pcz EMDB-17596, PDB-8pcz: Ligand-free SpSLC9C1 in lipid nanodiscs, dimer 手法: EM (単粒子) / 解像度: 3.21 Å 17598 8pd2 EMDB-17598, PDB-8pd2: Ligand-free SpSLC9C1 in lipid nanodiscs, protomer state 1 手法: EM (単粒子) / 解像度: 3.25 Å 17599 8pd3 EMDB-17599, PDB-8pd3: Ligand-free SpSLC9C1 in lipid nanodiscs, protomer state 2 手法: EM (単粒子) / 解像度: 3.3 Å 17601 8pd5 EMDB-17601, PDB-8pd5: Ligand-free SpSLC9C1 in lipid nanodiscs, protomer state 3 手法: EM (単粒子) / 解像度: 3.4 Å 17602 8pd7 EMDB-17602, PDB-8pd7: Ligand-free SpSLC9C1 in lipid nanodiscs, protomer state 4 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-17603: Ligand-free SpSLC9C1 in detergent, asymmetric class 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-17604: Ligand-free SpSLC9C1 in detergent, symmetric class 手法: EM (単粒子) / 解像度: 3.05 Å 17605 8pd8 EMDB-17605, PDB-8pd8: cAMP-bound SpSLC9C1 in lipid nanodiscs, dimer 手法: EM (単粒子) / 解像度: 3.3 Å 17607 8pd9 EMDB-17607, PDB-8pd9: cAMP-bound SpSLC9C1 in lipid nanodiscs, protomer state 1 手法: EM (単粒子) / 解像度: 3.6 Å 17621 8pdu EMDB-17621, PDB-8pdu: cGMP-bound SpSLC9C1 in lipid nanodiscs, dimer 手法: EM (単粒子) / 解像度: 3.22 Å 17622 8pdv EMDB-17622, PDB-8pdv: cGMP-bound SpSLC9C1 in lipid nanodiscs, protomer 手法: EM (単粒子) / 解像度: 3.26 Å EMDB-17625: cAMP-bound SpSLC9C1 in lipid nanodiscs, protomer state 2 手法: EM (単粒子) / 解像度: 3.74 Å
化合物	ChemComp-CMP: ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE / cAMP ChemComp-PCG: CYCLIC GUANOSINE MONOPHOSPHATE / cGMP
由来	strongylocentrotus purpuratus (ムラサキウニ)
キーワード	MEMBRANE PROTEIN / SLC9 / NHE / sperm-specific