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-Structure paper
タイトル | A marine cryptochrome with an inverse photo-oligomerization mechanism. |
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ジャーナル・号・ページ | Nat Commun, Vol. 14, Issue 1, Page 6918, Year 2023 |
掲載日 | 2023年10月30日 |
![]() | Hong Ha Vu / Heide Behrmann / Maja Hanić / Gayathri Jeyasankar / Shruthi Krishnan / Dennis Dannecker / Constantin Hammer / Monika Gunkel / Ilia A Solov'yov / Eva Wolf / Elmar Behrmann / ![]() |
PubMed 要旨 | Cryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, ...Cryptochromes (CRYs) are a structurally conserved but functionally diverse family of proteins that can confer unique sensory properties to organisms. In the marine bristle worm Platynereis dumerilii, its light receptive cryptochrome L-CRY (PdLCry) allows the animal to discriminate between sunlight and moonlight, an important requirement for synchronizing its lunar cycle-dependent mass spawning. Using cryo-electron microscopy, we show that in the dark, PdLCry adopts a dimer arrangement observed neither in plant nor insect CRYs. Intense illumination disassembles the dimer into monomers. Structural and functional data suggest a mechanistic coupling between the light-sensing flavin adenine dinucleotide chromophore, the dimer interface, and the C-terminal tail helix, with a likely involvement of the phosphate binding loop. Taken together, our work establishes PdLCry as a CRY protein with inverse photo-oligomerization with respect to plant CRYs, and provides molecular insights into how this protein might help discriminating the different light intensities associated with sunlight and moonlight. |
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手法 | EM (単粒子) |
解像度 | 2.57 - 8.0 Å |
構造データ | EMDB-17429, PDB-8p4x: ![]() EMDB-17533: FAD_AQ bound blue-light state structure of PdLCry |
化合物 | ![]() ChemComp-FAD: ![]() ChemComp-MG: ![]() ChemComp-HOH: |
由来 |
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![]() | FLAVOPROTEIN / light-sensitive / circalunar clock |