EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy.
ジャーナル・号・ページ	Nature, Vol. 466, Issue 7304, Page 329-333, Year 2010
掲載日	2010年7月15日
著者	Niels Fischer / Andrey L Konevega / Wolfgang Wintermeyer / Marina V Rodnina / Holger Stark /
PubMed 要旨	The translocation step of protein synthesis entails large-scale rearrangements of the ribosome-transfer RNA (tRNA) complex. Here we have followed tRNA movement through the ribosome during ...The translocation step of protein synthesis entails large-scale rearrangements of the ribosome-transfer RNA (tRNA) complex. Here we have followed tRNA movement through the ribosome during translocation by time-resolved single-particle electron cryomicroscopy (cryo-EM). Unbiased computational sorting of cryo-EM images yielded 50 distinct three-dimensional reconstructions, showing the tRNAs in classical, hybrid and various novel intermediate states that provide trajectories and kinetic information about tRNA movement through the ribosome. The structures indicate how tRNA movement is coupled with global and local conformational changes of the ribosome, in particular of the head and body of the small ribosomal subunit, and show that dynamic interactions between tRNAs and ribosomal residues confine the path of the tRNAs through the ribosome. The temperature dependence of ribosome dynamics reveals a surprisingly flat energy landscape of conformational variations at physiological temperature. The ribosome functions as a Brownian machine that couples spontaneous conformational changes driven by thermal energy to directed movement.
リンク	Nature / PubMed:20631791
手法	EM (単粒子)
解像度	9.0 - 20.0 Å
構造データ	EMDB-1716: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-1717: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-1718: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-1719: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 13.0 Å EMDB-1720: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-1721: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-1722: Ribosome dynamics and tRNA movement as visualized by time-resolved electon cryomicroscopy 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-1723: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-1724: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-1725: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 14.0 Å EMDB-1726: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 11.0 Å EMDB-1727: Ribosome dynamics and tRNA movement as visualized by time-resolved electron cryomicroscopy 手法: EM (単粒子) / 解像度: 18.0 Å
由来	Escherichia coli (大腸菌) synthetic construct (人工物)