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-Structure paper
タイトル | Structural changes in a marine podovirus associated with release of its genome into Prochlorococcus. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 17, Issue 7, Page 830-836, Year 2010 |
掲載日 | 2010年6月13日 |
著者 | Xiangan Liu / Qinfen Zhang / Kazuyoshi Murata / Matthew L Baker / Matthew B Sullivan / Caroline Fu / Matthew T Dougherty / Michael F Schmid / Marcia S Osburne / Sallie W Chisholm / Wah Chiu / |
PubMed 要旨 | Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of ...Podovirus P-SSP7 infects Prochlorococcus marinus, the most abundant oceanic photosynthetic microorganism. Single-particle cryo-electron microscopy yields icosahedral and asymmetrical structures of infectious P-SSP7 with 4.6-A and 9-A resolution, respectively. The asymmetric reconstruction reveals how symmetry mismatches are accommodated among five of the gene products at the portal vertex. Reconstructions of infectious and empty particles show a conformational change of the 'valve' density in the nozzle, an orientation difference in the tail fibers, a disordering of the C terminus of the portal protein and the disappearance of the core proteins. In addition, cryo-electron tomography of P-SSP7 infecting Prochlorococcus showed the same tail-fiber conformation as that in empty particles. Our observations suggest a mechanism whereby, upon binding to the host cell, the tail fibers induce a cascade of structural alterations of the portal vertex complex that triggers DNA release. |
リンク | Nat Struct Mol Biol / PubMed:20543830 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (単粒子) |
解像度 | 4.6 - 24.0 Å |
構造データ | EMDB-1707: EMDB-1713, PDB-2xd8: EMDB-1714: EMDB-1715: |
由来 |
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キーワード | VIRUS / MARINE PODOVIRUS / T7-LIKE VIRUS |