EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.
ジャーナル・号・ページ	Science, Vol. 326, Issue 5958, Page 1369-1373, Year 2009
掲載日	2009年12月4日
著者	Thomas Becker / Shashi Bhushan / Alexander Jarasch / Jean-Paul Armache / Soledad Funes / Fabrice Jossinet / James Gumbart / Thorsten Mielke / Otto Berninghausen / Klaus Schulten / Eric Westhof / Reid Gilmore / Elisabet C Mandon / Roland Beckmann /
PubMed 要旨	The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may ...The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.
リンク	Science / PubMed:19933108 / PubMed Central
手法	EM (単粒子)
解像度	6.1 - 8.9 Å
構造データ	EMDB-1651: Cryo-EM structure of the programmed yeast 80 ribosome bound the Ssh1 complex 手法: EM (単粒子) / 解像度: 6.1 Å 1652 2wwb EMDB-1652: Cryo-EM structure of the mammalian Sec61 complex bound to the actively translating wheat germ 80S ribosome PDB-2wwb: CRYO-EM STRUCTURE OF THE MAMMALIAN SEC61 COMPLEX BOUND TO THE ACTIVELY TRANSLATING WHEAT GERM 80S RIBOSOME 手法: EM (単粒子) / 解像度: 6.48 Å 1667 2ww9 EMDB-1667: Cryo-EM structure of the active yeast Ssh1 complex bound to the programmed yeast 80S ribosome bearing a P-site tRNA PDB-2ww9: Cryo-EM structure of the active yeast Ssh1 complex bound to the yeast 80S ribosome 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-1668: Cryo-EM structure of the active yeast 80S ribosome bearing a P-site tRNA and with the rRNA expansion segment ES27 in the exit conformation 手法: EM (単粒子) / 解像度: 7.9 Å 1669 2wwa EMDB-1669: Cryo-EM structures of the idle yeast Ssh1 complex bound to the yeast 80S ribosome PDB-2wwa: Cryo-EM structure of idle yeast Ssh1 complex bound to the yeast 80S ribosome 手法: EM (単粒子) / 解像度: 8.8 Å
由来	saccharomyces cerevisiae (パン酵母) canis lupus familiaris (イヌ) triticum aestivum (コムギ)
キーワード	RIBOSOME / RIBOSOMAL PROTEIN / RIBONUCLEOPROTEIN / TRANSMEMBRANE / PHOSPHOPROTEIN / SIGNAL SEQUENCE / MEMBRANE / TRANSPORT / RNA-BINDING / RRNA-BINDING / TRANSLOCATION / PROTEIN CONDUCTING CHANNEL / PROTEIN EXIT TUNNEL / ENDOPLASMIC RETICULUM / COTRANSLATIONAL PROTEIN TRANSLOCATION / ISOPEPTIDE BOND / PROTEIN TRANSPORT