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-Structure paper
タイトル | Morphological Diversity of Dps Complex with Genomic DNA. |
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ジャーナル・号・ページ | Int J Mol Sci, Vol. 24, Issue 10, Year 2023 |
掲載日 | 2023年5月10日 |
著者 | Yuri Chesnokov / Roman Kamyshinsky / Andrey Mozhaev / Eleonora Shtykova / Alexander Vasiliev / Ivan Orlov / Liubov Dadinova / |
PubMed 要旨 | In response to adverse environmental factors, cells actively produce Dps proteins which form ordered complexes (biocrystals) with bacterial DNA to protect the genome. The effect of ...In response to adverse environmental factors, cells actively produce Dps proteins which form ordered complexes (biocrystals) with bacterial DNA to protect the genome. The effect of biocrystallization has been described extensively in the scientific literature; furthermore, to date, the structure of the Dps-DNA complex has been established in detail in vitro using plasmid DNA. In the present work, for the first time, Dps complexes with genomic DNA were studied in vitro using cryo-electron tomography. We demonstrate that genomic DNA forms one-dimensional crystals or filament-like assemblies which transform into weakly ordered complexes with triclinic unit cells, similar to what is observed for plasmid DNA. Changing such environmental factors as pH and KCl and MgCl concentrations leads to the formation of cylindrical structures. |
リンク | Int J Mol Sci / PubMed:37239879 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 22.0 - 30.5 Å |
構造データ | EMDB-16438: Toroidal Dps-DNA assembly EMDB-16439: Dps-DNA filament-like assembly |
由来 |
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