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-Structure paper
タイトル | A Bayesian approach to single-particle electron cryo-tomography in RELION-4.0. |
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ジャーナル・号・ページ | Elife, Vol. 11, Year 2022 |
掲載日 | 2022年12月5日 |
著者 | Jasenko Zivanov / Joaquín Otón / Zunlong Ke / Andriko von Kügelgen / Euan Pyle / Kun Qu / Dustin Morado / Daniel Castaño-Díez / Giulia Zanetti / Tanmay A M Bharat / John A G Briggs / Sjors H W Scheres / |
PubMed 要旨 | We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are ...We present a new approach for macromolecular structure determination from multiple particles in electron cryo-tomography (cryo-ET) data sets. Whereas existing subtomogram averaging approaches are based on 3D data models, we propose to optimise a regularised likelihood target that approximates a function of the 2D experimental images. In addition, analogous to Bayesian polishing and contrast transfer function (CTF) refinement in single-particle analysis, we describe the approaches that exploit the increased signal-to-noise ratio in the averaged structure to optimise tilt-series alignments, beam-induced motions of the particles throughout the tilt-series acquisition, defoci of the individual particles, as well as higher-order optical aberrations of the microscope. Implementation of our approaches in the open-source software package RELION aims to facilitate their general use, particularly for those researchers who are already familiar with its single-particle analysis tools. We illustrate for three applications that our approaches allow structure determination from cryo-ET data to resolutions sufficient for de novo atomic modelling. |
リンク | Elife / PubMed:36468689 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 3.0 - 3.8 Å |
構造データ | EMDB-15949: COPII inner coat reprocessed with relion4.0 EMDB-16183, PDB-8bqe: EMDB-16207: HIV-1 CA-SP1 subtomogram average with Relion4 from EMPIAR-10164 dataset, 3.2 A from 5 tomograms EMDB-16209: HIV-1 CA-SP1 subtomogram average with Relion4 from EMPIAR-10164 dataset, 3.0 A from the full dataset |
化合物 | ChemComp-CA: ChemComp-MG: ChemComp-GNP: |
由来 |
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キーワード | STRUCTURAL PROTEIN / RsaA S-layer sub-tomogram averaging Caulobacter / PROTEIN TRANSPORT / COPII |