EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a volume-regulated heteromeric LRRC8A/C channel.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 1, Page 52-61, Year 2023
掲載日	2022年12月15日
著者	Sonja Rutz / Dawid Deneka / Antje Dittmann / Marta Sawicka / Raimund Dutzler /
PubMed 要旨	Volume-regulated anion channels (VRACs) participate in the cellular response to osmotic swelling. These membrane proteins consist of heteromeric assemblies of LRRC8 subunits, whose compositions ...Volume-regulated anion channels (VRACs) participate in the cellular response to osmotic swelling. These membrane proteins consist of heteromeric assemblies of LRRC8 subunits, whose compositions determine permeation properties. Although structures of the obligatory LRRC8A, also referred to as SWELL1, have previously defined the architecture of VRACs, the organization of heteromeric channels has remained elusive. Here we have addressed this question by the structural characterization of murine LRRC8A/C channels. Like LRRC8A, these proteins assemble as hexamers. Despite 12 possible arrangements, we find a predominant organization with an A:C ratio of two. In this assembly, four LRRC8A subunits cluster in their preferred conformation observed in homomers, as pairs of closely interacting proteins that stabilize a closed state of the channel. In contrast, the two interacting LRRC8C subunits show a larger flexibility, underlining their role in the destabilization of the tightly packed A subunits, thereby enhancing the activation properties of the protein.
リンク	Nat Struct Mol Biol / PubMed:36522427 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.1 - 18.2 Å
構造データ	15835 8b40 EMDB-15835: Cryo-EM structure of homomeric LRRC8C Volume-Regulated Anion Channel PDB-8b40: Structure of homomeric LRRC8C Volume-Regulated Anion Channel 手法: EM (単粒子) / 解像度: 4.6 Å 15836 8b41 EMDB-15836: Cryo-EM structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 PDB-8b41: Structure of heteromeric LRRC8A/C (1:1 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 手法: EM (単粒子) / 解像度: 3.8 Å 15837 8b42 EMDB-15837: Cryo-EM structure of heteromeric LRRC8A/C Volume-Regulated Anion Channel PDB-8b42: Structure of heteromeric LRRC8A/C Volume-Regulated Anion Channel. 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-15838: Cryo-EM structure of heteromeric LRRC8A/C (1:3 co-transfected) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 手法: EM (単粒子) / 解像度: 9.5 Å EMDB-15839: Cryo-EM structure of heteromeric LRRC8A/C (endogenous) Volume-Regulated Anion Channel in complex with synthetic nanobody Sb1 手法: EM (単粒子) / 解像度: 18.2 Å EMDB-15840: Cryo-EM structure of homomeric LRRC8A_SAM Volume-Regulated Anion Channel 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-15841: Cryo-EM structure of heteromeric LRRC8A_SAM/C Volume-Regulated Anion Channel 手法: EM (単粒子) / 解像度: 7.8 Å PDB-8ben: LRR domain Structure of the LRRC8C protein 手法: X-RAY DIFFRACTION / 解像度: 3.1 Å
由来	mus musculus (ハツカネズミ) synthetic construct (人工物) Homo sapiens (ヒト)
キーワード	MEMBRANE PROTEIN / Ion channel / Volume-regulated anion channel