EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural plasticity of bacterial ESCRT-III protein PspA in higher-order assemblies.
ジャーナル・号・ページ	Nat Struct Mol Biol, Year 2024
掲載日	2024年8月16日
著者	Benedikt Junglas / Esther Hudina / Philipp Schönnenbeck / Ilona Ritter / Anja Heddier / Beatrix Santiago-Schübel / Pitter F Huesgen / Dirk Schneider / Carsten Sachse /
PubMed 要旨	Eukaryotic members of the endosome sorting complex required for transport-III (ESCRT-III) family have been shown to form diverse higher-order assemblies. The bacterial phage shock protein A (PspA) ...Eukaryotic members of the endosome sorting complex required for transport-III (ESCRT-III) family have been shown to form diverse higher-order assemblies. The bacterial phage shock protein A (PspA) has been identified as a member of the ESCRT-III superfamily, and PspA homo-oligomerizes to form rod-shaped assemblies. As observed for eukaryotic ESCRT-III, PspA forms tubular assemblies of varying diameters. Using electron cryo-electron microscopy, we determined 61 Synechocystis PspA structures and observed in molecular detail how the structural plasticity of PspA rods is mediated by conformational changes at three hinge regions in the monomer and by the fixed and changing molecular contacts between protomers. Moreover, we reduced and increased the structural plasticity of PspA rods by removing the loop connecting helices α3/α4 and the addition of nucleotides, respectively. Based on our analysis of PspA-mediated membrane remodeling, we suggest that the observed mode of structural plasticity is a prerequisite for the biological function of ESCRT-III members.
リンク	Nat Struct Mol Biol / PubMed:39152237
手法	EM (らせん対称)
解像度	3.8 - 6.9 Å
構造データ	15489 8akq EMDB-15489, PDB-8akq: 180 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 4.1 Å 15490 8akr EMDB-15490, PDB-8akr: 200 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 3.8 Å 15491 8aks EMDB-15491, PDB-8aks: 215 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 3.9 Å 15492 8akt EMDB-15492, PDB-8akt: 235 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 4.4 Å 15493 8aku EMDB-15493, PDB-8aku: 250 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 4.5 Å 15494 8akv EMDB-15494, PDB-8akv: 270 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 4.4 Å 15495 8akw EMDB-15495, PDB-8akw: 280 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 5.4 Å 15496 8akx EMDB-15496, PDB-8akx: 305 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 6.6 Å 15497 8aky EMDB-15497, PDB-8aky: 290 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 4.7 Å 15498 8akz EMDB-15498, PDB-8akz: 320 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 6.9 Å 15499 8al0 EMDB-15499, PDB-8al0: 365 A SynPspA rod after incubation with ATP 手法: EM (らせん対称) / 解像度: 6.9 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM
由来	synechocystis sp. pcc 6803 (バクテリア)
キーワード	LIPID BINDING PROTEIN / Nucleotide binding / Helical assembly / ESCRT-III fold / Membrane remodeling