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-Structure paper
| タイトル | Visualization of the hybrid state of tRNA binding promoted by spontaneous ratcheting of the ribosome. |
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| ジャーナル・号・ページ | Mol Cell, Vol. 32, Issue 2, Page 190-197, Year 2008 |
| 掲載日 | 2008年10月24日 |
著者 | Xabier Agirrezabala / Jianlin Lei / Julie L Brunelle / Rodrigo F Ortiz-Meoz / Rachel Green / Joachim Frank / ![]() |
| PubMed 要旨 | A crucial step in translation is the translocation of tRNAs through the ribosome. In the transition from one canonical site to the other, the tRNAs acquire intermediate configurations, so-called ...A crucial step in translation is the translocation of tRNAs through the ribosome. In the transition from one canonical site to the other, the tRNAs acquire intermediate configurations, so-called hybrid states. At this stage, the small subunit is rotated with respect to the large subunit, and the anticodon stem loops reside in the A and P sites of the small subunit, while the acceptor ends interact with the P and E sites of the large subunit. In this work, by means of cryo-EM and particle classification procedures, we visualize the hybrid state of both A/P and P/E tRNAs in an authentic factor-free ribosome complex during translocation. In addition, we show how the repositioning of the tRNAs goes hand in hand with the change in the interplay between S13, L1 stalk, L5, H68, H69, and H38 that is caused by the ratcheting of the small subunit. |
リンク | Mol Cell / PubMed:18951087 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 8.9 - 10.5 Å |
| 構造データ | ![]() EMDB-1540: ![]() EMDB-1541: |
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