EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Filament structure and subcellular organization of the bacterial intermediate filament-like protein crescentin.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 121, Issue 7, Page e2309984121, Year 2024
掲載日	2024年2月13日
著者	Yue Liu / Fusinita van den Ent / Jan Löwe /
PubMed 要旨	The protein crescentin is required for the crescent shape of the freshwater bacterium (). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features ...The protein crescentin is required for the crescent shape of the freshwater bacterium (). Crescentin forms a filamentous structure on the inner, concave side of the curved cells. It shares features with eukaryotic intermediate filament (IF) proteins, including the formation of static filaments based on long and parallel coiled coils, the protein's length, structural roles in cell and organelle shape determination and the presence of a coiled coil discontinuity called the "stutter." Here, we have used electron cryomicroscopy (cryo-EM) to determine the structure of the full-length protein and its filament, exploiting a crescentin-specific nanobody. The filament is formed by two strands, related by twofold symmetry, that each consist of two dimers, resulting in an octameric assembly. Crescentin subunits form longitudinal contacts head-to-head and tail-to-tail, making the entire filament non-polar. Using in vivo site-directed cysteine cross-linking, we demonstrated that contacts observed in the in vitro filament structure exist in cells. Electron cryotomography (cryo-ET) of cells expressing crescentin showed filaments on the concave side of the curved cells, close to the inner membrane, where they form a band. When comparing with current models of IF proteins and their filaments, which are also built from parallel coiled coil dimers and lack overall polarity, it emerges that IF proteins form head-to-tail longitudinal contacts in contrast to crescentin and hence several inter-dimer contacts in IFs have no equivalents in crescentin filaments. Our work supports the idea that intermediate filament-like proteins achieve their shared polymerization and mechanical properties through a variety of filament architectures.
リンク	Proc Natl Acad Sci U S A / PubMed:38324567 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 6.9 Å
構造データ	15395 8afe EMDB-15395, PDB-8afe: Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and small box) 手法: EM (単粒子) / 解像度: 3.3 Å 15398 8afh EMDB-15398, PDB-8afh: Cryo-EM structure of crescentin filaments (stutter mutant, C2, symmetry and small box) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-15399: Cryo-EM map of crescentin filament in complex with a megabody (stutter mutant, C2 symmetry, large box) 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-15400: Cryo-EM map of crescentin filaments in complex with a megabody (wildtype, C2, large box) 手法: EM (単粒子) / 解像度: 6.9 Å 15401 8afl EMDB-15401, PDB-8afl: Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and small box) 手法: EM (単粒子) / 解像度: 4.4 Å 15402 8afm EMDB-15402, PDB-8afm: Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and small box) 手法: EM (単粒子) / 解像度: 4.8 Å 15446 8ahl EMDB-15446, PDB-8ahl: Cryo-EM structure of crescentin filaments (stutter mutant, C1 symmetry and large box) 手法: EM (単粒子) / 解像度: 4.1 Å 15465 8aia EMDB-15465, PDB-8aia: Cryo-EM structure of crescentin filaments (wildtype, C1 symmetry and large box) 手法: EM (単粒子) / 解像度: 5.1 Å 15473 8aix EMDB-15473, PDB-8aix: Cryo-EM structure of crescentin filaments (wildtype, C2 symmetry and large box) 手法: EM (単粒子) / 解像度: 5.8 Å 15476 8ajb EMDB-15476, PDB-8ajb: Cryo-EM structure of crescentin filaments (stutter mutant, C2 symmetry and large box) 手法: EM (単粒子) / 解像度: 4.3 Å
由来	caulobacter vibrioides (バクテリア) camelidae (哺乳類)
キーワード	STRUCTURAL PROTEIN / cytoskeleton / cell shape / intermediate filaments / coiled coil / assembly