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-Structure paper
タイトル | The 3D structure of villin as an unusual F-Actin crosslinker. |
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ジャーナル・号・ページ | Structure, Vol. 16, Issue 12, Page 1882-1891, Year 2008 |
掲載日 | 2008年12月10日 |
![]() | Cheri M Hampton / Jun Liu / Dianne W Taylor / David J DeRosier / Kenneth A Taylor / ![]() |
PubMed 要旨 | Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate ...Villin is an F-actin nucleating, crosslinking, severing, and capping protein within the gelsolin superfamily. We have used electron tomography of 2D arrays of villin-crosslinked F-actin to generate 3D images revealing villin's crosslinking structure. In these polar arrays, neighboring filaments are spaced 125.9 +/- 7.1 A apart, offset axially by 17 A, with one villin crosslink per actin crossover. More than 6500 subvolumes containing a single villin crosslink and the neighboring actin filaments were aligned and classified to produce 3D subvolume averages. Placement of a complete villin homology model into the average density reveals that full-length villin binds to different sites on F-actin from those used by other actin-binding proteins and villin's close homolog gelsolin. |
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手法 | EM (トモグラフィー) |
解像度 | 30.0 Å |
構造データ | ![]() EMDB-1491: ![]() EMDB-1496: ![]() EMDB-1497: ![]() EMDB-1537: ![]() EMDB-1538: ![]() EMDB-1539: |
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