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-Structure paper
タイトル | Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts. |
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ジャーナル・号・ページ | Commun Biol, Vol. 7, Issue 1, Page 557, Year 2024 |
掲載日 | 2024年5月10日 |
![]() | Lisa Schmidt / Christian Tüting / Fotis L Kyrilis / Farzad Hamdi / Dmitry A Semchonok / Gerd Hause / Annette Meister / Christian Ihling / Milton T Stubbs / Andrea Sinz / Panagiotis L Kastritis / ![]() ![]() |
PubMed 要旨 | The high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to ...The high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to investigate. Here, we retrieve cell extracts enriched with an endogenous virus, the yeast L-A virus. The determined cryo-EM structure discloses capsid-stabilizing cation-π stacking, widespread across viruses and within the Totiviridae, and an interplay of non-covalent interactions from ten distinct capsomere interfaces. The capsid-embedded mRNA decapping active site trench is supported by a constricting movement of two flexible opposite-facing loops. tRNA-loaded polysomes and other biomacromolecules, presumably mRNA, are found in virus proximity within the cell extract. Mature viruses participate in larger viral communities resembling their rare in-cell equivalents in terms of size, composition, and inter-virus distances. Our results collectively describe a 3D-architecture of a viral milieu, opening the door to cell-extract-based high-resolution structural virology. |
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手法 | EM (単粒子) |
解像度 | 3.21 - 8.1 Å |
構造データ | EMDB-15189, PDB-8a5t: ![]() EMDB-15214: Endogenous yeast L-A helper virus identified from native cell extracts ![]() EMDB-15215: Asymmetric reconstruction of averaged ribosomes from Saccharomyces cerevisiae EMDB-17628, PDB-8pe4: |
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![]() | VIRUS / Capsid structure ScVLA / viral particle / wildtype / endogenous |