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-Structure paper
タイトル | Insight into DNA and protein transport in double-stranded DNA viruses: the structure of bacteriophage N4. |
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ジャーナル・号・ページ | J Mol Biol, Vol. 378, Issue 3, Page 726-736, Year 2008 |
掲載日 | 2008年5月2日 |
著者 | Kyung H Choi / Jennifer McPartland / Irene Kaganman / Valorie D Bowman / Lucia B Rothman-Denes / Michael G Rossmann / |
PubMed 要旨 | Bacteriophage N4 encapsidates a 3500-aa-long DNA-dependent RNA polymerase (vRNAP), which is injected into the host along with the N4 genome upon infection. The three-dimensional structures of wild- ...Bacteriophage N4 encapsidates a 3500-aa-long DNA-dependent RNA polymerase (vRNAP), which is injected into the host along with the N4 genome upon infection. The three-dimensional structures of wild-type and mutant N4 viruses lacking gp17, gp50, or gp65 were determined by cryoelectron microscopy. The virion has an icosahedral capsid with T=9 quasi-symmetry that encapsidates well-organized double-stranded DNA and vRNAP. The tail, attached at a unique pentameric vertex of the head, consists of a neck, 12 appendages, and six ribbons that constitute a non-contractile sheath around a central tail tube. Comparison of wild-type and mutant virus structures in conjunction with bioinformatics established the identity and virion locations of the major capsid protein (gp56), a decorating protein (gp17), the vRNAP (gp50), the tail sheath (gp65), the appendages (gp66), and the portal protein (gp59). The N4 virion organization provides insight into its assembly and suggests a mechanism for genome and vRNAP transport strategies utilized by this unique system. |
リンク | J Mol Biol / PubMed:18374942 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 14.0 - 30.0 Å |
構造データ | EMDB-1472: EMDB-1475: EMDB-1476: EMDB-1509: |